• Immunohistochemistry analysis of paraffin-embedded mouse brain using RPN1 antibody (STJ114371) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using RPN1 antibody (STJ114371) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using RPN1 antibody (STJ114371) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using RPN1 antibody (STJ114371) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RPN1 antibody (20-280) (STJ114371)

SKU:
STJ114371

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RPN1 (20-280) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPN1
Gene ID: 6184
Uniprot ID: RPN1_HUMAN
Immunogen Region: 20-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-280 of human RPN1 (NP_002941.1).
Immunogen Sequence: APGSASSEAPPLINEDVKRT VDLSSHLAKVTAEVVLAHLG GGSTSRATSFLLALEPELEA RLAHLGVQVKGEDEEENNLE VRETKIKGKSGRFFTVKLPV ALDPGAKISVIVETVYTHVL HPYPTQITQSEKQFVVFEGN HYFYSPYPTKTQTMRVKLAS RNVESYTKLGNPTRSEDLLD YGPFRDVPAYSQDTFKVHYE NNSPFLTITSMTRVIEVSHW GNIAVEENVDLKHTGAVLK
Tissue Specificity Expressed in all tissues tested.
Post Translational Modifications Ufmylated by UFL1 in response to endoplasmic reticulum stress, promoting reticulophagy of endoplasmic reticulum sheets.
Function Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
Protein Name Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase Subunit 1
Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase 67 Kda Subunit
Ribophorin I
Rpn-I
Ribophorin-1
Database Links Reactome: R-HSA-1799339
Reactome: R-HSA-446203
Reactome: R-HSA-9694548
Cellular Localisation Endoplasmic Reticulum
Endoplasmic Reticulum Membrane
Single-Pass Type I Membrane Protein
Melanosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase Subunit 1 antibody
Anti-Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase 67 Kda Subunit antibody
Anti-Ribophorin I antibody
Anti-Rpn-I antibody
Anti-Ribophorin-1 antibody
Anti-RPN1 antibody

Information sourced from Uniprot.org

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