• Immunohistochemistry analysis of paraffin-embedded mouse brain using RPL4 Rabbit polyclonal antibody (STJ29925) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using RPL4 Rabbit polyclonal antibody (STJ29925) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using RPL4 antibody (STJ29925) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-RPL4 antibody (1-240) (STJ29925)

SKU:
STJ29925

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RPL4 (1-240) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPL4
Gene ID: 6124
Uniprot ID: RL4_HUMAN
Immunogen Region: 1-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human RPL4 (NP_000959.2).
Immunogen Sequence: MACARPLISVYSEKGESSGK NVTLPAVFKAPIRPDIVNFV HTNLRKNNRQPYAVSELAGH QTSAESWGTGRAVARIPRVR GGGTHRSGQGAFGNMCRGGR MFAPTKTWRRWHRRVNTTQK RYAICSALAASALPALVMSK GHRIEEVPELPLVVEDKVEG YKKTKEAVLLLKKLKAWNDI KKVYASQRMRAGKGKMRNRR RIQRRGPCIIYNEDNGIIKA FRNIPGITLLNVSKLNILK
Post Translational Modifications Citrullinated by PADI4.
Function Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.
Protein Name Large Ribosomal Subunit Protein Ul4
60s Ribosomal Protein L1
60s Ribosomal Protein L4
Database Links Reactome: R-HSA-156827
Reactome: R-HSA-156902
Reactome: R-HSA-1799339
Reactome: R-HSA-192823
Reactome: R-HSA-2408557
Reactome: R-HSA-6791226
Reactome: R-HSA-72689
Reactome: R-HSA-72706
Reactome: R-HSA-72764
Reactome: R-HSA-9010553
Reactome: R-HSA-9633012
Reactome: R-HSA-975956
Reactome: R-HSA-975957
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Large Ribosomal Subunit Protein Ul4 antibody
Anti-60s Ribosomal Protein L1 antibody
Anti-60s Ribosomal Protein L4 antibody
Anti-RPL4 antibody
Anti-RPL1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance