![Western blot analysis of lysates from wild type (WT) and RPL22 knockout (KO) 293T cells, using [KO Validated] RPL22 Rabbit polyclonal antibody (STJ11100742) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90S.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89680/358173/STJ11100742_1__83977.1713122480.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and RPL22 knockout (KO) 293T cells, using [KO Validated] RPL22 Rabbit polyclonal antibody (STJ11100742) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90S.")
![Western blot analysis of lysates from wild type (WT) and RPL22 knockout (KO) 293T cells, using [KO Validated] RPL22 Rabbit polyclonal antibody (STJ11100742) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90S.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89680/358173/STJ11100742_1__83977.1713122480.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and RPL22 knockout (KO) 293T cells, using [KO Validated] RPL22 Rabbit polyclonal antibody (STJ11100742) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90S.")
Anti-RPL22 antibody (1-122) (STJ11100742)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RPL22 (1-122) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:3000IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RPL22 |
| Gene ID | 6146 |
| Uniprot ID | RL22_HUMAN |
| Immunogen Region | 1-122 |
| Immunogen Sequence | MAPVKKLVVKGGKKKKQVLK FTLDCTHPVEDGIMDAANFE QFLQERIKVNGKAGNLGGGV VTIERSKSKITVTSEVPFSK RYLKYLTKKYLKKNNLRDWL RVVANSKESYELRYFQINQD EE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-122 of human RPL22 (NP_000974.1). |
Additional Info
| Function | Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. |
| Protein Name | Large Ribosomal Subunit Protein El2260s Ribosomal Protein L22Eber-Associated ProteinEapEpstein-Barr Virus Small Rna-Associated ProteinHeparin-Binding Protein Hbp15 |
| Database Links | Reactome: R-HSA-156827Reactome: R-HSA-156902Reactome: R-HSA-1799339Reactome: R-HSA-192823Reactome: R-HSA-2408557Reactome: R-HSA-6791226Reactome: R-HSA-72689Reactome: R-HSA-72706Reactome: R-HSA-72764Reactome: R-HSA-9010553Reactome: R-HSA-9633012Reactome: R-HSA-975956Reactome: R-HSA-975957 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Large Ribosomal Subunit Protein El22 antibodyAnti-60s Ribosomal Protein L22 antibodyAnti-Eber-Associated Protein antibodyAnti-Eap antibodyAnti-Epstein-Barr Virus Small Rna-Associated Protein antibodyAnti-Heparin-Binding Protein Hbp15 antibodyAnti-RPL22 antibody |
Information sourced from Uniprot.org