| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:100-1:500ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RPL13A |
| Gene ID: | 23521 |
| Uniprot ID: | RL13A_HUMAN |
| Immunogen Region: | 1-197 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-197 of human RPL13A (NP_036555.1). |
| Immunogen Sequence: | MAEVQVLVLDGRGHLLGRLA AIVAKQVLLGRKVVVVRCEG INISGNFYRNKLKYLAFLRK RMNTNPSRGPYHFRAPSRIF WRTVRGMLPHKTKRGQAALD RLKVFDGIPPPYDKKKRMVV PAALKVVRLKPTRKFAYLGR LAHEVGWKYQAVTATLEEKR KEKAKIHYRKKKQLMRLRKQ AEKNVEKKIDKYTEVLK |
| Post Translational Modifications | Phosphorylation at Ser-77 upon interferon-gamma treatment in monocytes involves a DAPK1-DAPK3 kinase cascade and is causing release from the ribosome, association with the GAIT complex and subsequent involvement in transcript-selective translation inhibition. Citrullinated by PADI4. |
| Function | Associated with ribosomes but is not required for canonical ribosome function and has extra-ribosomal functions. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation and subsequent phosphorylation dissociates from the ribosome and assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. In the GAIT complex interacts with m7G cap-bound eIF4G at or near the eIF3-binding site and blocks the recruitment of the 43S ribosomal complex. Involved in methylation of rRNA. |
| Protein Name | Large Ribosomal Subunit Protein Ul1323 Kda Highly Basic Protein60s Ribosomal Protein L13a |
| Database Links | Reactome: R-HSA-156827Reactome: R-HSA-156902Reactome: R-HSA-1799339Reactome: R-HSA-192823Reactome: R-HSA-2408557Reactome: R-HSA-6791226Reactome: R-HSA-72689Reactome: R-HSA-72706Reactome: R-HSA-72764Reactome: R-HSA-9010553Reactome: R-HSA-9633012Reactome: R-HSA-975956Reactome: R-HSA-975957 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Large Ribosomal Subunit Protein Ul13 antibodyAnti-23 Kda Highly Basic Protein antibodyAnti-60s Ribosomal Protein L13a antibodyAnti-RPL13A antibody |
Information sourced from Uniprot.org
