• Western blot analysis of extracts of various cell lines, using RPL13A antibody (STJ11104667) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-RPL13A antibody (1-197) (STJ11104667)

SKU:
STJ11104667

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Large Ribosomal Subunit Protein Ul13 (1-197) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPL13A
Gene ID: 23521
Uniprot ID: RL13A_HUMAN
Immunogen Region: 1-197
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-197 of human RPL13A (NP_036555.1).
Immunogen Sequence: MAEVQVLVLDGRGHLLGRLA AIVAKQVLLGRKVVVVRCEG INISGNFYRNKLKYLAFLRK RMNTNPSRGPYHFRAPSRIF WRTVRGMLPHKTKRGQAALD RLKVFDGIPPPYDKKKRMVV PAALKVVRLKPTRKFAYLGR LAHEVGWKYQAVTATLEEKR KEKAKIHYRKKKQLMRLRKQ AEKNVEKKIDKYTEVLK
Post Translational Modifications Phosphorylation at Ser-77 upon interferon-gamma treatment in monocytes involves a DAPK1-DAPK3 kinase cascade and is causing release from the ribosome, association with the GAIT complex and subsequent involvement in transcript-selective translation inhibition. Citrullinated by PADI4.
Function Associated with ribosomes but is not required for canonical ribosome function and has extra-ribosomal functions. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation and subsequent phosphorylation dissociates from the ribosome and assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. In the GAIT complex interacts with m7G cap-bound eIF4G at or near the eIF3-binding site and blocks the recruitment of the 43S ribosomal complex. Involved in methylation of rRNA.
Protein Name Large Ribosomal Subunit Protein Ul13
23 Kda Highly Basic Protein
60s Ribosomal Protein L13a
Database Links Reactome: R-HSA-156827
Reactome: R-HSA-156902
Reactome: R-HSA-1799339
Reactome: R-HSA-192823
Reactome: R-HSA-2408557
Reactome: R-HSA-6791226
Reactome: R-HSA-72689
Reactome: R-HSA-72706
Reactome: R-HSA-72764
Reactome: R-HSA-9010553
Reactome: R-HSA-9633012
Reactome: R-HSA-975956
Reactome: R-HSA-975957
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Large Ribosomal Subunit Protein Ul13 antibody
Anti-23 Kda Highly Basic Protein antibody
Anti-60s Ribosomal Protein L13a antibody
Anti-RPL13A antibody

Information sourced from Uniprot.org

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