• Immunofluorescence analysis of MCF-7 cells using RPL13 antibody (STJ28806). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using RPL13 Antibody (STJ28806) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat spinal cord using RPL13 Antibody (STJ28806) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using RPL13 antibody (STJ28806) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-RPL13 antibody (1-211) (STJ28806)

SKU:
STJ28806

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RPL13 (1-211) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPL13
Gene ID: 6137
Uniprot ID: RL13_HUMAN
Immunogen Region: 1-211
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human RPL13 (NP_150254.1).
Immunogen Sequence: MAPSRNGMVLKPHFHKDWQR RVATWFNQPARKIRRRKARQ AKARRIAPRPASGPIRPIVR CPTVRYHTKVRAGRGFSLEE LRVAGIHKKVARTIGISVDP RRRNKSTESLQANVQRLKEY RSKLILFPRKPSAPKKGDSS AEELKLATQLTGPVMPVRNV YKKEKARVITEEEKNFKAFA SLRMARANARLFGIRAKRAK EAAEQDVEKKK
Tissue Specificity Higher levels of expression in benign breast lesions than in carcinomas.
Function Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules (Probable). The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain (Probable). The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel (Probable). As part of the LSU, it is probably required for its formation and the maturation of rRNAs. Plays a role in bone development.
Protein Name Large Ribosomal Subunit Protein El13
60s Ribosomal Protein L13
Breast Basic Conserved Protein 1
Database Links Reactome: R-HSA-156827
Reactome: R-HSA-156902
Reactome: R-HSA-1799339
Reactome: R-HSA-192823
Reactome: R-HSA-2408557
Reactome: R-HSA-6791226
Reactome: R-HSA-72689
Reactome: R-HSA-72706
Reactome: R-HSA-72764
Reactome: R-HSA-9010553
Reactome: R-HSA-9633012
Reactome: R-HSA-975956
Reactome: R-HSA-975957
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Large Ribosomal Subunit Protein El13 antibody
Anti-60s Ribosomal Protein L13 antibody
Anti-Breast Basic Conserved Protein 1 antibody
Anti-RPL13 antibody
Anti-BBC1 antibody
Anti-OK antibody
Anti-SW-cl.46 antibody

Information sourced from Uniprot.org

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