| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S8MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:1000-1:2000 IHC-P:1:200-1:800 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ROCK2 |
| Gene ID: | 9475 |
| Uniprot ID: | ROCK2_HUMAN |
| Immunogen Region: | 1289-1388 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 1289-1388 of human ROCK2 (O75116). |
| Immunogen Sequence: | PALECRRCHIKCHKDHMDKK EEIIAPCKVYYDISTAKNLL LLANSTEEQQKWVSRLVKKI PKKPPAPDPFARSSPRTSMK IQQNQSIRRPSRQLAPNKPS |
| Tissue Specificity | Expressed in the brain (at protein level). |
| Post Translational Modifications | Phosphorylation at Tyr-722 reduces its binding to RHOA and is crucial for focal adhesion dynamics. Dephosphorylation by PTPN11 stimulates its RHOA binding activity. Cleaved by granzyme B during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing. |
| Function | Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation. |
| Protein Name | Rho-Associated Protein Kinase 2 Rho Kinase 2 Rho-Associated - Coiled-Coil-Containing Protein Kinase 2 Rho-Associated - Coiled-Coil-Containing Protein Kinase Ii Rock-Ii P164 Rock-2 |
| Database Links | Reactome: R-HSA-3928662 Reactome: R-HSA-3928663 Reactome: R-HSA-416482 Reactome: R-HSA-416572 Reactome: R-HSA-4420097 Reactome: R-HSA-5627117 Reactome: R-HSA-8980692 Reactome: R-HSA-9013026 Reactome: R-HSA-9013106 Reactome: R-HSA-9013407 Reactome: R-HSA-9013422 Reactome: R-HSA-9679191 |
| Cellular Localisation | Cytoplasm Cell Membrane Peripheral Membrane Protein Nucleus Cytoskeleton Microtubule Organizing Center Centrosome Cytoplasmic And Associated With Actin Microfilaments And The Plasma Membrane |
| Alternative Antibody Names | Anti-Rho-Associated Protein Kinase 2 antibody Anti-Rho Kinase 2 antibody Anti-Rho-Associated - Coiled-Coil-Containing Protein Kinase 2 antibody Anti-Rho-Associated - Coiled-Coil-Containing Protein Kinase Ii antibody Anti-Rock-Ii antibody Anti-P164 Rock-2 antibody Anti-ROCK2 antibody Anti-KIAA0619 antibody |
Information sourced from Uniprot.org