| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:100 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ROCK1 |
| Gene ID: | 6093 |
| Uniprot ID: | ROCK1_HUMAN |
| Immunogen Region: | 400-660 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human ROCK1 (NP_005397.1). |
| Immunogen Sequence: | YSNRRYLSSANPNDNRTSSN ADKSLQESLQKTIYKLEEQL HNEMQLKDEMEQKCRTSNIK LDKIMKELDEEGNQRRNLES TVSQIEKEKMLLQHRINEYQ RKAEQENEKRRNVENEVSTL KDQLEDLKKVSQNSQLANEK LSQLQKQLEEANDLLRTESD TAVRLRKSHTEMSKSISQLE SLNRELQERNRILENSKSQT DKDYYQLQAILEAERRDRGH DSEMIGDLQARITSLQEEV |
| Tissue Specificity | Detected in blood platelets. |
| Post Translational Modifications | Autophosphorylated on serine and threonine residues. Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing. |
| Function | Protein kinase which is a key regulator of the actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, TPPP, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. Inhibits podocyte motility via regulation of actin cytoskeletal dynamics and phosphorylation of CFL1. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. |
| Protein Name | Rho-Associated Protein Kinase 1 Renal Carcinoma Antigen Ny-Ren-35 Rho-Associated - Coiled-Coil-Containing Protein Kinase 1 Rho-Associated - Coiled-Coil-Containing Protein Kinase I Rock-I P160 Rock-1 P160rock |
| Database Links | Reactome: R-HSA-111465 Reactome: R-HSA-3928662 Reactome: R-HSA-3928663 Reactome: R-HSA-416482 Reactome: R-HSA-416572 Reactome: R-HSA-4420097 Reactome: R-HSA-5627117 Reactome: R-HSA-6798695 Reactome: R-HSA-8980692 Reactome: R-HSA-9013026 Reactome: R-HSA-9013106 Reactome: R-HSA-9013407 Reactome: R-HSA-9013422 Reactome: R-HSA-9679191 Reactome: R-HSA-9696264 |
| Cellular Localisation | Cytoplasm Cytoskeleton Microtubule Organizing Center Centrosome Centriole Golgi Apparatus Membrane Peripheral Membrane Protein Cell Projection Bleb Cell Membrane Lamellipodium Ruffle A Small Proportion Is Associated With Golgi Membranes Associated With The Mother Centriole And An Intercentriolar Linker Colocalizes With Itgb1bp1 And Itgb1 At The Cell Membrane Predominantly In Lamellipodia And Membrane Ruffles But Also In Retraction Fibers Localizes At The Cell Membrane In An Itgb1bp1-Dependent Manner |
| Alternative Antibody Names | Anti-Rho-Associated Protein Kinase 1 antibody Anti-Renal Carcinoma Antigen Ny-Ren-35 antibody Anti-Rho-Associated - Coiled-Coil-Containing Protein Kinase 1 antibody Anti-Rho-Associated - Coiled-Coil-Containing Protein Kinase I antibody Anti-Rock-I antibody Anti-P160 Rock-1 antibody Anti-P160rock antibody Anti-ROCK1 antibody |
Information sourced from Uniprot.org