| Host: | Rabbit |
| Applications: | IHC/WB |
| Reactivity: | Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal anti-Robo1 (C-Term) for use in IHC and WB in Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Lyophilised |
| Purification: | IgG purified |
| Dilution Range: | IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: | Robo1 |
| Gene ID: | 19876 |
| Uniprot ID: | ROBO1_MOUSE |
| Immunogen Region: | C-Term |
| Specificity: | Specific for Robo1. |
| Immunogen: | A synthetic peptide to from c-terminal region of mouse Robo1 (Roundabout homolog 1) conjugated to an immunogenic carrier protein was used as the antigen. |
| Post Translational Modifications | Ubiquitinated. May be deubiquitinated by USP33. |
| Function | Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. Plays a role in the regulation of cell migration via its interaction with MYO9B.inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA. May be required for lung development. |
| Protein Name | Roundabout Homolog 1 |
| Database Links | Reactome: R-MMU-376176Reactome: -MMU-428890Reactome: -MMU-8985586Reactome: -MMU-9010553 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCell ProjectionAxonEndoplasmic Reticulum-Golgi Intermediate Compartment MembraneSingle-Pass Membrane ProteinDetected At Growth Cones In Thalamus NeuronsPrrg4 Prevents Cell Surface Location And Both Colocalize In The Endoplasmic Reticulum/Golgi Adjacent To The Cell Nucleus |
| Alternative Antibody Names | Anti-Roundabout Homolog 1 antibodyAnti-Robo1 antibodyAnti-Dutt1 antibody |
Information sourced from Uniprot.org
