| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ROBO1 (1452-1651) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:200-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ROBO1 |
| Gene ID: |
6091 |
| Uniprot ID: |
ROBO1_HUMAN |
| Immunogen Region: |
1452-1651 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1452-1651 of human ROBO1 (NP_002932.1). |
| Immunogen Sequence: |
AAVMQKTRPAKKLKHQPGHL RRETYTDDLPPPPVPPPAIK SPTAQSKTQLEVRPVVVPKL PSMDARTDRSSDRKGSSYKG REVLDGRQVVDMRTNPGDPR EAQEQQNDGKGRGNKAAKRD LPPAKTHLIQEDILPYCRPT FPTSNNPRDPSSSSSMSSRG SGSRQREQANVGRRNIAEMQ VLGGYERGEDNNEELEETES |
| Tissue Specificity | Widely expressed, with exception of kidney. |
| Post Translational Modifications | Ubiquitinated. May be deubiquitinated by USP33. |
| Function | Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. Plays a role in the regulation of cell migration via its interaction with MYO9B.inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA. May be required for lung development. |
| Protein Name | Roundabout Homolog 1Deleted In U Twenty TwentyH-Robo-1 |
| Database Links | Reactome: R-HSA-373752Reactome: R-HSA-376176Reactome: R-HSA-428540Reactome: R-HSA-428542Reactome: R-HSA-428543Reactome: R-HSA-428890Reactome: R-HSA-8985586Reactome: R-HSA-8985801Reactome: R-HSA-9010553 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCell ProjectionAxonEndoplasmic Reticulum-Golgi Intermediate Compartment MembraneSingle-Pass Membrane ProteinDetected At Growth Cones In Thalamus NeuronsPrrg4 Prevents Cell Surface Location And Both Colocalize In The Endoplasmic Reticulum/Golgi Adjacent To The Cell Nucleus |
| Alternative Antibody Names | Anti-Roundabout Homolog 1 antibodyAnti-Deleted In U Twenty Twenty antibodyAnti-H-Robo-1 antibodyAnti-ROBO1 antibodyAnti-DUTT1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
