Anti-RNF40 antibody (231-431) [S0MR] (STJ11102500)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal RNF40 (231-431) antibody for use in WB, IHC-P and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S0MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RNF40 |
| Gene ID | 9810 |
| Uniprot ID | BRE1B_HUMAN |
| Immunogen Region | 231-431 |
| Immunogen Sequence | RELGRENRRLQDLATQLQEK HHRISLEYSELQDKVTSAET KVLEMETTVEDLQWDIEKLR KREQKLNKHLAEALEQLNSG YYVSGSSSGFQGGQITLSMQ KFEMLNAELEENQELANSRM AELEKLQAELQGAVRTNERL KVALRSLPEEVVRETGEYRM LQAQFSLLYNESLQVKTQLD EARGLLLATKNSHLRHIEHM E |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 231-431 of human RNF40 (O75150). |
Additional Info
| Tissue Specificity | Ubiquitously expressed. Expressed at higher level in testis, heart and pancreas, while it is only weakly expressed in lung, skeletal muscle and small intestine. |
| Function | Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B.reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. (Microbial infection) Promotes the human herpesvirus 8 (KSHV) lytic cycle by inducing the expression of lytic viral genes including the latency switch gene RTA/ORF50. |
| Protein Name | E3 Ubiquitin-Protein Ligase Bre1bBre1-B95 Kda Retinoblastoma-Associated ProteinRbp95Ring Finger Protein 40Ring-Type E3 Ubiquitin Transferase Bre1b |
| Database Links | Reactome: R-HSA-8866654 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Bre1b antibodyAnti-Bre1-B antibodyAnti-95 Kda Retinoblastoma-Associated Protein antibodyAnti-Rbp95 antibodyAnti-Ring Finger Protein 40 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Bre1b antibodyAnti-RNF40 antibodyAnti-BRE1B antibodyAnti-KIAA0661 antibody |
Information sourced from Uniprot.org