| Host: |
Goat |
| Applications: |
Pep-ELISA/WB/IHC |
| Reactivity: |
Human/Mouse/Rat/Dog/Pig/Cow |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-RNF36/TRIM69 (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 55kDa band observed in Mouse Testis lysates (calculated MW of 57.3kDa according to Mouse NP_536771.2). Recommended concentration: 0.5-2µg/ml.IHC: Paraffin embedded Human Te |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
TRIM69 |
| Gene ID: |
140691 |
| Uniprot ID: |
TRI69_HUMAN |
| Immunogen Region: |
C-Term |
| Accession Number: |
NP_892030.3; NP_542783.2 |
| Specificity: |
This antibody is expected to recognize both reported isoforms (NP_892030.3; NP_542783.2). |
| Immunogen Sequence: |
GENKEPLHILHPQ |
| Function | E3 ubiquitin ligase that plays an important role in antiviral immunity by restricting different viral infections including dengue virus or vesicular stomatitis indiana virus. Ubiquitinates viral proteins such as dengue virus NS3 thereby limiting infection. In addition, acts as a key mediator of type I interferon induced microtubule stabilization by directly associating to microtubules independently of its E3 ligase activity. Plays also a role in cataract formation together with TP53. Mechanistically, inhibits UVB-induced cell apoptosis and reactive oxygen species (ROS) production by inducing TP53 ubiquitination. Regulates centrosome dynamics and mitotic progression by ubiquitinating STK3/MST2.leading to its redistribution to the perinuclear cytoskeleton and subsequent phosphorylation by PLK1. |
| Protein Name | E3 Ubiquitin-Protein Ligase Trim69Rfp-Like Domain-Containing Protein TrimlessRing Finger Protein 36Ring-Type E3 Ubiquitin Transferase Trim69Tripartite Motif-Containing Protein 69 |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmNucleusNucleus SpeckleCytoskeletonMicrotubule Organizing CenterCentrosomeAdopts A Filamentous Distribution In The Cell Cytoplasm Where It Strongly Colocalizes With Stable Microtubules |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Trim69 antibodyAnti-Rfp-Like Domain-Containing Protein Trimless antibodyAnti-Ring Finger Protein 36 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Trim69 antibodyAnti-Tripartite Motif-Containing Protein 69 antibodyAnti-TRIM69 antibodyAnti-RNF36 antibodyAnti-HSD-34 antibodyAnti-HSD34 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
