Anti-RNF213/C17orf27 antibody (Internal) (STJ71092)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-RNF213/C17orf27 (Internal) is suitable for use in ELISA and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/IHC |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Not yet tested-our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 374kDa according to NP_065965.3. Therefore we canno |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | RNF213 |
| Gene ID | 57674 |
| Uniprot ID | RN213_HUMAN |
| Accession Number | NP_065965.3 |
| Immunogen Region | Internal |
| Immunogen Sequence | KNPQTQTEERFRPQ |
Additional Info
| Post Translational Modifications | Autoubiquitinated. |
| Function | Atypical E3 ubiquitin ligase that can catalyze ubiquitination of both proteins and lipids, and which is involved in various processes, such as lipid metabolism, angiogenesis and cell-autonomous immunity. Acts as a key immune sensor by catalyzing ubiquitination of the lipid A moiety of bacterial lipopolysaccharide (LPS) via its RZ-type zinc-finger: restricts the proliferation of cytosolic bacteria, such as Salmonella, by generating the bacterial ubiquitin coat through the ubiquitination of LPS. Also acts indirectly by mediating the recruitment of the LUBAC complex, which conjugates linear polyubiquitin chains. Ubiquitination of LPS triggers cell-autonomous immunity, such as antibacterial autophagy, leading to degradation of the microbial invader. Involved in lipid metabolism by regulating fat storage and lipid droplet formation.act by inhibiting the lipolytic process. Also regulates lipotoxicity by inhibiting desaturation of fatty acids. Also acts as an E3 ubiquitin-protein ligase via its RING-type zinc finger: mediates 'Lys-63'-linked ubiquitination of target proteins. Involved in the non-canonical Wnt signaling pathway in vascular development: acts by mediating ubiquitination and degradation of FLNA and NFATC2 downstream of RSPO3, leading to inhibit the non-canonical Wnt signaling pathway and promoting vessel regression. Also has ATPase activity.ATPase activity is required for ubiquitination of LPS. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf213Alk Lymphoma Oligomerization Partner On Chromosome 17E3 Ubiquitin-Lipopolysaccharide Ligase Rnf213MysterinRing Finger Protein 213 |
| Database Links | Reactome: R-HSA-9635465Reactome: R-HSA-9725370Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmCytosolLipid Droplet |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf213 antibodyAnti-Alk Lymphoma Oligomerization Partner On Chromosome 17 antibodyAnti-E3 Ubiquitin-Lipopolysaccharide Ligase Rnf213 antibodyAnti-Mysterin antibodyAnti-Ring Finger Protein 213 antibodyAnti-RNF213 antibodyAnti-ALO17 antibodyAnti-C17orf27 antibodyAnti-KIAA1554 antibodyAnti-KIAA1618 antibodyAnti-MYSTR antibody |
Information sourced from Uniprot.org