• Chromatin immunoprecipitation analysis of extracts of HeLa cells, using RING1B/RNF2 antibody (STJ27509) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Immunoprecipitation analysis of 200 Mu g extracts of 293T cells using 1 Mu g RING1B/RNF2 antibody (STJ27509). Western blot was performed from the immunoprecipitate using RING1B/RNF2 antibody (STJ27509) at a dilution of 1:1000.
  • Immunohistochemistry analysis of RING1B/RNF2 in paraffin-embedded rat kidney using RING1B/RNF2 Rabbit polyclonal antibody (STJ27509) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of RING1B/RNF2 in paraffin-embedded mouse kidney using RING1B/RNF2 Rabbit polyclonal antibody (STJ27509) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of RING1B/RNF2 in paraffin-embedded human liver cancer using RING1B/RNF2 Rabbit polyclonal antibody (STJ27509) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Jurkat cells, using RING1B/RNF2 Rabbit polyclonal antibody (STJ27509) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-RNF2 antibody (1-336) (STJ27509)

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STJ27509

€140.63 - €653.13
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RING1B/RNF2 (1-336) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:50-1:200
ChIP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RNF2
Gene ID: 6045
Uniprot ID: RING2_HUMAN
Immunogen Region: 1-336
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-336 of human RING1B/RING1B/RNF2 (NP_009143.1).
Immunogen Sequence: MSQAVQTNGTQPLSKTWELS LYELQRTPQEAITDGLEIVV SPRSLHSELMCPICLDMLKN TMTTKECLHRFCADCIITAL RSGNKECPTCRKKLVSKRSL RPDPNFDALISKIYPSRDEY EAHQERVLARINKHNNQQAL SHSIEEGLKIQAMNRLQRGK KQQIENGSGAEDNGDSSHCS NASTHSNQEAGPSNKRTKTS DDSGLELDNNNAAMAIDPVM DGASEIELVFRPHPTLMEK
Post Translational Modifications Monoubiquitinated, by auto-ubiquitination. Polyubiquitinated in the presence of UBE2D3 (in vitro).
Function E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A (H2AK119Ub), thereby playing a central role in histone code and gene regulation. H2AK119Ub gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity (Probable). Association with the chromosomal DNA is cell-cycle dependent. In resting B- and T-lymphocytes, interaction with AURKB leads to block its activity, thereby maintaining transcription in resting lymphocytes. Also acts as a negative regulator of autophagy by mediating ubiquitination of AMBRA1, leading to its subsequent degradation.
Protein Name E3 Ubiquitin-Protein Ligase Ring2
Huntingtin-Interacting Protein 2-Interacting Protein 3
Hip2-Interacting Protein 3
Protein Ding
Ring Finger Protein 1b
Ring1b
Ring Finger Protein 2
Ring Finger Protein Bap-1
Ring-Type E3 Ubiquitin Transferase Ring2
Database Links Reactome: R-HSA-2559580
Reactome: R-HSA-3108214
Reactome: R-HSA-3899300
Reactome: R-HSA-4551638
Reactome: R-HSA-4570464
Reactome: R-HSA-4655427
Reactome: R-HSA-8939243
Reactome: R-HSA-8943724
Reactome: R-HSA-8953750
Cellular Localisation Nucleus
Cytoplasm
Chromosome
Enriched On Inactive X Chromosome (Xi) In Female Trophoblast Stem (Ts) Cells As Well As Differentiating Embryonic Stem (Es) Cells
The Enrichment On Xi Is Transient During Ts And Es Cell Differentiation
The Association With Xi Is Mitotically Stable In Non-Differentiated Ts Cells
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Ring2 antibody
Anti-Huntingtin-Interacting Protein 2-Interacting Protein 3 antibody
Anti-Hip2-Interacting Protein 3 antibody
Anti-Protein Ding antibody
Anti-Ring Finger Protein 1b antibody
Anti-Ring1b antibody
Anti-Ring Finger Protein 2 antibody
Anti-Ring Finger Protein Bap-1 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Ring2 antibody
Anti-RNF2 antibody
Anti-BAP1 antibody
Anti-DING antibody
Anti-HIPI3 antibody
Anti-RING1B antibody

Information sourced from Uniprot.org

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