| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RNF185 |
| Gene ID: | 91445 |
| Uniprot ID: | RN185_HUMAN |
| Immunogen Region: | 1-130 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human RNF185 (NP_689480.2). |
| Immunogen Sequence: | MASKGPSASASPENSSAGGP SGSSNGAGESGGQDSTFECN ICLDTAKDAVISLCGHLFCW PCLHQWLETRPNRQVCPVCK AGISRDKVIPLYGRGSTGQQ DPREKTPPRPQGQRPEPENR GGFQGFGFGD |
| Tissue Specificity | Ubiquitously expressed. |
| Function | E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating 'Lys-63'-linked polyubiquitination of BNIP1. Acts in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway, which targets misfolded proteins that accumulate in the endoplasmic reticulum (ER) for ubiquitination and subsequent proteasome-mediated degradation. Protects cells from ER stress-induced apoptosis. Responsible for the cotranslational ubiquitination and degradation of CFTR in the ERAD pathway. Also acts as a regulator of the innate antiviral response by catalyzing 'Lys-27'-linked polyubiquitination of CGAS at 'Lys-173' and 'Lys-384', thereby promoting CGAS cyclic GMP-AMP synthase activity. Preferentially associates with the E2 enzymes UBE2J1 and UBE2J2. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf185 Ring Finger Protein 185 |
| Database Links | Reactome: R-HSA-382556 Reactome: R-HSA-5678895 Reactome: R-HSA-901032 |
| Cellular Localisation | Mitochondrion Outer Membrane Multi-Pass Membrane Protein Endoplasmic Reticulum Membrane |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf185 antibody Anti-Ring Finger Protein 185 antibody Anti-RNF185 antibody |
Information sourced from Uniprot.org