| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RNF185 |
| Gene ID: |
91445 |
| Uniprot ID: |
RN185_HUMAN |
| Immunogen Region: |
1-130 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human RNF185 (NP_689480.2). |
| Immunogen Sequence: |
MASKGPSASASPENSSAGGP SGSSNGAGESGGQDSTFECN ICLDTAKDAVISLCGHLFCW PCLHQWLETRPNRQVCPVCK AGISRDKVIPLYGRGSTGQQ DPREKTPPRPQGQRPEPENR GGFQGFGFGD |
| Tissue Specificity | Ubiquitously expressed. |
| Function | E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating 'Lys-63'-linked polyubiquitination of BNIP1. Acts in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway, which targets misfolded proteins that accumulate in the endoplasmic reticulum (ER) for ubiquitination and subsequent proteasome-mediated degradation. Protects cells from ER stress-induced apoptosis. Responsible for the cotranslational ubiquitination and degradation of CFTR in the ERAD pathway. Also acts as a regulator of the innate antiviral response by catalyzing 'Lys-27'-linked polyubiquitination of CGAS at 'Lys-173' and 'Lys-384', thereby promoting CGAS cyclic GMP-AMP synthase activity. Preferentially associates with the E2 enzymes UBE2J1 and UBE2J2. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf185Ring Finger Protein 185 |
| Database Links | Reactome: R-HSA-382556Reactome: R-HSA-5678895Reactome: R-HSA-901032 |
| Cellular Localisation | Mitochondrion Outer MembraneMulti-Pass Membrane ProteinEndoplasmic Reticulum Membrane |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf185 antibodyAnti-Ring Finger Protein 185 antibodyAnti-RNF185 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
