Western blot analysis of various lysates using RNF180 Rabbit polyclonal antibody (STJ117435) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-RNF180 antibody (1-416) (STJ117435)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ117435
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General Information

Short DescriptionRabbit polyclonal RNF180 (1-416) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolRNF180
Gene ID285671
Uniprot IDRN180_HUMAN
Immunogen Region1-416
Immunogen SequenceMKRSKELITKNHSQEETSIL RCWKCRKCIASSGCFMEYLE NQVIKDKDDSVDAQNICHVW HMNVEALPEWISCLIQKAQW TVGKLNCPFCGARLGGFNFV STPKCSCGQLAAVHLSKSRT DYQPTQAGRLMRPSVKYLSH PRVQSGCDKEALLTGGGSEN RNHRLLNMARNNNDPGRLTE ALCLEVRPTYFEMKNEKLLS KASEPKYQLFVPQLVTGRCA TRAFHRKSHSLDLNISEKL
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 1-416 of human RNF180 (NP_848627.1).

Additional Info

Function E3 ubiquitin-protein ligase which promotes polyubiquitination and degradation by the proteasome pathway of ZIC2.
Protein Name E3 Ubiquitin-Protein Ligase Rnf180
Ring Finger Protein 180
Ring-Type E3 Ubiquitin Transferase Rnf180
Database Links
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Membrane Protein
Nucleus Envelope
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Rnf180 antibody
Anti-Ring Finger Protein 180 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Rnf180 antibody
Anti-RNF180 antibody

Information sourced from Uniprot.org

Citations

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