| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RNF180 |
| Gene ID: | 285671 |
| Uniprot ID: | RN180_HUMAN |
| Immunogen Region: | 1-416 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-416 of human RNF180 (NP_848627.1). |
| Immunogen Sequence: | MKRSKELITKNHSQEETSIL RCWKCRKCIASSGCFMEYLE NQVIKDKDDSVDAQNICHVW HMNVEALPEWISCLIQKAQW TVGKLNCPFCGARLGGFNFV STPKCSCGQLAAVHLSKSRT DYQPTQAGRLMRPSVKYLSH PRVQSGCDKEALLTGGGSEN RNHRLLNMARNNNDPGRLTE ALCLEVRPTYFEMKNEKLLS KASEPKYQLFVPQLVTGRCA TRAFHRKSHSLDLNISEKL |
| Function | E3 ubiquitin-protein ligase which promotes polyubiquitination and degradation by the proteasome pathway of ZIC2. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf180Ring Finger Protein 180Ring-Type E3 Ubiquitin Transferase Rnf180 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Membrane ProteinNucleus Envelope |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf180 antibodyAnti-Ring Finger Protein 180 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Rnf180 antibodyAnti-RNF180 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and Caspase-9 Rabbit polyclonal antibody knockout (KO) 293T cells, using [KO Validated] Caspase-9 Rabbit polyclonal antibody (STJ11104477) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/177121/368484/STJ11104477_1__01028.1713133967.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Caspase-9 Rabbit polyclonal antibody knockout (KO) 293T cells, using [KO Validated] Caspase-9 Rabbit polyclonal antibody (STJ11104477) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
