• Western blot analysis of various lysates using RNF152 Rabbit polyclonal antibody (STJ119035) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-RNF152 antibody (100-200) (STJ119035)

SKU:
STJ119035

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RNF152 (100-200) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RNF152
Gene ID: 220441
Uniprot ID: RN152_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human RNF152 (NP_775828.1).
Immunogen Sequence: YMLPLPISKERALLPGDMGC RLLPGSQQKSVTVVTIPAEQ QPLQGGAPQEAVEEEQDRRG VVKSSTWSGVCTVILVACVL VFLLGIVLHNMSCISKRFTV I
Tissue Specificity Widely expressed.
Post Translational Modifications Ubiquitinated. Autoubiquitinated in vitro, leading to its degradation by the proteasome (Probable).
Function E3 ubiquitin-protein ligase that acts as a negative regulator of mTORC1 signaling by mediating ubiquitination of RagA/RRAGA and RHEB. Catalyzes 'Lys-63'-linked polyubiquitination of RagA/RRAGA in response to amino acid starvation, thereby regulating mTORC1 signaling. Also mediates monoubiquitination of RHEB, promoting its association with the TSC-TBC complex and subsequent inhibition. Also mediates 'Lys-48'-linked polyubiquitination of target proteins and their subsequent targeting to the proteasome for degradation. Induces apoptosis when overexpressed.
Protein Name E3 Ubiquitin-Protein Ligase Rnf152
Ring Finger Protein 152
Ring-Type E3 Ubiquitin Transferase Rnf152
Database Links Reactome: R-HSA-8866654
Cellular Localisation Lysosome Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Rnf152 antibody
Anti-Ring Finger Protein 152 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Rnf152 antibody
Anti-RNF152 antibody

Information sourced from Uniprot.org

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