Anti-RNF144B antibody (Internal) (STJ94909)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the Internal region of human p53RFP.
General Information
| Short Description | Rabbit polyclonal anti-E3 ubiquitin-protein ligase RNF144B (Internal) for use in WB and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000ELISA 1:40000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RNF144B |
| Gene ID | 255488 |
| Uniprot ID | R144B_HUMAN |
| Immunogen | Synthesized peptide derived from the Internal region of human p53RFP. |
| Immunogen Region | Internal |
| Specificity | p53RFP Polyclonal Antibody detects endogenous levels of p53RFP protein. |
Additional Info
| Post Translational Modifications | Auto-ubiquitinated. |
| Function | E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. Plays a crucial role in maintaining the genomic stability by controlling the degradation of multiple proteins involved in mitotic progression and DNA damage. Regulates epithelial homeostasis by mediating degradation of CDKN1A and isoform 2 of TP63. Plays a regulatory role in innate immunity by negatively regulating IRF3 activation and IFN-beta production. Mechanistically, inhibits TBK1 phosphorylation and 'Lys-63'-linked polyubiquitination independently of its E3 ligase activity. Alternatively, promotes 'Lys-27' and 'Lys-33'-linked ubiquitination of IFIH1/MDA5, promoting selective autophagic degradation of IFIH1/MDA5 to inhibit antiviral response. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf144bIbr Domain-Containing Protein 2Ring Finger Protein 144bP53-Inducible Ring Finger Protein |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | Mitochondrion MembraneSingle-Pass Membrane ProteinCytoplasmMostly CytosololicAccumulates In Submitochondrial Domains Specifically Upon Apoptosis InductionIn Synchrony With Bax Activation |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf144b antibodyAnti-Ibr Domain-Containing Protein 2 antibodyAnti-Ring Finger Protein 144b antibodyAnti-P53-Inducible Ring Finger Protein antibodyAnti-RNF144B antibodyAnti-IBRDC2 antibodyAnti-P53RFP antibody |
Information sourced from Uniprot.org