| Short Description : | Goat polyclonal antibody anti-RNF13/RZF-Isoform 1/3 (C-Term) is suitable for use in ELISA, Western Blot, Immunohistochemistry and Flow Cytometry research applications. |
| Applications: | Pep-ELISA/WB/IHC/FC |
| Host: | Goat |
| Reactivity: | Human/Mouse/Rat/Dog/Pig/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | 0.5 mg/mL |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:1000.WB: Approx. 42kDa band observed in lysates of cell line A431 and in preliminary testing of Human Lung lysate, and approx. 45kDa in lysates of cell line HeLa and in preliminary testing of U2 |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | RNF13 |
| Gene ID: | 11342 |
| Uniprot ID: | RNF13_HUMAN |
| Accession Number: | NP_009213.1 |
| Immunogen Region: | C-Term |
| Immunogen Sequence: | PNGERDYNIANTV |
| Specificity: | Reported variants represent identical protein (NP_009213.1; NP_899237.1). |
| Post Translational Modifications | Autoubiquitinated. |
| Function | E3 ubiquitin-protein ligase that regulates cell proliferation. Involved in apoptosis regulation. Mediates ER stress-induced activation of JNK signaling pathway and apoptosis by promoting ERN1 activation and splicing of XBP1 mRNA. Also involved in protein trafficking and localization. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf13Ring Finger Protein 13 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type I Membrane ProteinLate Endosome MembraneLysosome MembraneNucleus Inner MembraneUnder Certain ConditionsRelocalizes To Recycling Endosomes And To The Inner Nuclear Membrane |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf13 antibodyAnti-Ring Finger Protein 13 antibodyAnti-RNF13 antibodyAnti-RZF antibody |
Information sourced from Uniprot.org
