| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RNF115 |
| Gene ID: |
27246 |
| Uniprot ID: |
RN115_HUMAN |
| Immunogen Region: |
30-140 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 30-140 of human RNF115 (NP_055270.1). |
| Immunogen Sequence: |
SPKLPEYICPRCESGFIEEV TDDSSFLGGGGSRIDNTTTT HFAELWGHLDHTMFFQDFRP FLSSSPLDQDNRANERGHQT HTDFWGARPPRLPLGRRYRS RGSSRPDRSPA |
| Tissue Specificity | Expressed at extremely low levels in normal breast, prostate, lung, colon. Higher levels of expression are detected in heart, skeletal muscle, testis as well as in breast and prostate cancer cells. |
| Post Translational Modifications | Phosphorylated by AKT1, allowing association with the 14-3-3 chaperones that facilitates associating with TLRs. RING-type zinc finger-dependent and E2-dependent autoubiquitination. Deubiquitinated by USP9X.antogonizing its autoubiquitination and subsequent proteasomal degradation. |
| Function | E3 ubiquitin-protein ligase that catalyzes the 'Lys-48'- and/or 'Lys-63'-linked polyubiquitination of various substrates and thereby plays a role in a number of signaling pathways including autophagy, innate immunity, cell proliferation and cell death. Plays a role in the endosomal trafficking and degradation of membrane receptors including EGFR, FLT3, MET and CXCR4 through their polyubiquitination. Participates together with BST2 in antiviral immunity by facilitating the internalization of HIV-1 virions into intracellular vesicles leading to their lysosomal degradation. Possesses also an antiviral activity independently of BST2 by promoting retroviral GAG proteins ubiquitination, redistribution to endo-lysosomal compartments and, ultimately, lysosomal degradation. Catalyzes distinct types of ubiquitination on MAVS and STING1 at different phases of viral infection to promote innate antiviral response. Mediates the 'Lys-48'-linked ubiquitination of MAVS leading to its proteasomal degradation and ubiquitinates STING1 via 'Lys-63'-linked polyubiquitination, critical for its oligomerization and the subsequent recruitment of TBK1. Plays a positive role in the autophagosome-lysosome fusion by interacting with STX17 and enhancing its stability without affecting 'Lys-48'- or 'Lys-63'-linked polyubiquitination levels, which in turn promotes autophagosome maturation. Negatively regulates TLR-induced expression of proinflammatory cytokines by catalyzing 'Lys-11'-linked ubiquitination of RAB1A and RAB13 to inhibit post-ER trafficking of TLRs to the Golgi by RAB1A and subsequently from the Golgi apparatus to the cell surface by RAB13. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf115Ring Finger Protein 115Ring-Type E3 Ubiquitin Transferase Rnf115Rab7-Interacting Ring Finger ProteinRabring 7Zinc Finger Protein 364 |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmNucleusEndoplasmic ReticulumGolgi ApparatusThe Gtp-Bound Form Of Rab7a Recruits Rnf115 From The Cytosol Onto Late Endosomes/Lysosomes |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf115 antibodyAnti-Ring Finger Protein 115 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Rnf115 antibodyAnti-Rab7-Interacting Ring Finger Protein antibodyAnti-Rabring 7 antibodyAnti-Zinc Finger Protein 364 antibodyAnti-RNF115 antibodyAnti-ZNF364 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
