| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RNF114 (1-228) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RNF114 |
| Gene ID: |
55905 |
| Uniprot ID: |
RN114_HUMAN |
| Immunogen Region: |
1-228 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-228 of human RNF114 (NP_061153.1). |
| Immunogen Sequence: |
MAAQQRDCGGAAQLAGPAAE ADPLGRFTCPVCLEVYEKPV QVPCGHVFCSACLQECLKPK KPVCGVCRSALAPGVRAVEL ERQIESTETSCHGCRKNFFL SKIRSHVATCSKYQNYIMEG VKATIKDASLQPRNVPNRYT FPCPYCPEKNFDQEGLVEHC KLFHSTDTKSVVCPICASMP WGDPNYRSANFREHIQRRHR FSYDTFVDYDVDEEDMMNQV LQRSIIDQ |
| Tissue Specificity | Expressed in numerous tissues, including skin, CD4 lymphocytes and dendritic cells. Highest levels in testis. |
| Post Translational Modifications | Autoubiquitinated. Polyubiquitinated in the presence of E2 enzymes UBE2D1, UBE2D2 and UBE2D3, but only monoubiquitinated in the presence of UBE2E1. |
| Function | E3 ubiquitin-protein ligase that promotes the ubiquitination of various substrates. In turn, participates in the regulation of many biological processes including cell cycle, apoptosis, osteoclastogenesis as well as innate or adaptive immunity. Acts as negative regulator of NF-kappa-B-dependent transcription by promoting the ubiquitination and stabilization of the NF-kappa-B inhibitor TNFAIP3. May promote the ubiquitination of TRAF6 as well. Acts also as a negative regulator of T-cell activation. Inhibits cellular dsRNA responses and interferon production by targeting MAVS component for proteasomal degradation. Ubiquitinates the CDK inhibitor CDKN1A leading to its degradationand probably also CDKN1B and CDKN1C. This activity stimulates cell cycle G1-to-S phase transition and suppresses cellular senescence. May play a role in spermatogenesis. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf114Ring Finger Protein 114Ring-Type E3 Ubiquitin Transferase Rnf114Zinc Finger Protein 228Zinc Finger Protein 313 |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf114 antibodyAnti-Ring Finger Protein 114 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Rnf114 antibodyAnti-Zinc Finger Protein 228 antibodyAnti-Zinc Finger Protein 313 antibodyAnti-RNF114 antibodyAnti-ZNF228 antibodyAnti-ZNF313 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and CACYBP knockout (KO) 293T cells, using [KO Validated] CACYBP Rabbit polyclonal antibody (STJ11100880) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89814/358277/STJ11100880_1__41081.1713122624.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CACYBP knockout (KO) 293T cells, using [KO Validated] CACYBP Rabbit polyclonal antibody (STJ11100880) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
