| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RNASEH2A |
| Gene ID: | 10535 |
| Uniprot ID: | RNH2A_HUMAN |
| Immunogen Region: | 1-299 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-299 of human RNASEH2A (NP_006388.2). |
| Immunogen Sequence: | MDLSELERDNTGRCRLSSPV PAVCRKEPCVLGVDEAGRGP VLGPMVYAICYCPLPRLADL EALKVADSKTLLESERERLF AKMEDTDFVGWALDVLSPNL ISTSMLGRVKYNLNSLSHDT ATGLIQYALDQGVNVTQVFV DTVGMPETYQARLQQSFPGI EVTVKAKADALYPVVSAASI CAKVARDQAVKKWQFVEKLQ DLDTDYGSGYPNDPKTKAWL KEHVEPVFGFPQFVRFSWR |
| Function | Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes. |
| Protein Name | Ribonuclease H2 Subunit ARnase H2 Subunit AAicardi-Goutieres Syndrome 4 ProteinAgs4Rnase H(35Ribonuclease Hi Large SubunitRnase Hi Large SubunitRibonuclease Hi Subunit A |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Ribonuclease H2 Subunit A antibodyAnti-Rnase H2 Subunit A antibodyAnti-Aicardi-Goutieres Syndrome 4 Protein antibodyAnti-Ags4 antibodyAnti-Rnase H(35 antibodyAnti-Ribonuclease Hi Large Subunit antibodyAnti-Rnase Hi Large Subunit antibodyAnti-Ribonuclease Hi Subunit A antibodyAnti-RNASEH2A antibodyAnti-RNASEHI antibodyAnti-RNHIA antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and NHEJ1 knockout (KO) HeLa cells, using [KO Validated] NHEJ1 Rabbit polyclonal antibody (STJ11103937) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/176582/367429/STJ11103937_1__73085.1713132967.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and NHEJ1 knockout (KO) HeLa cells, using [KO Validated] NHEJ1 Rabbit polyclonal antibody (STJ11103937) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.")
