• Immunofluorescence analysis of C6 cells using RNASEH2A Rabbit polyclonal antibody (STJ117326) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using RNASEH2A Rabbit polyclonal antibody (STJ117326) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-RNASEH2A antibody (1-299) (STJ117326)

SKU:
STJ117326

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RNASEH2A (1-299) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RNASEH2A
Gene ID: 10535
Uniprot ID: RNH2A_HUMAN
Immunogen Region: 1-299
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-299 of human RNASEH2A (NP_006388.2).
Immunogen Sequence: MDLSELERDNTGRCRLSSPV PAVCRKEPCVLGVDEAGRGP VLGPMVYAICYCPLPRLADL EALKVADSKTLLESERERLF AKMEDTDFVGWALDVLSPNL ISTSMLGRVKYNLNSLSHDT ATGLIQYALDQGVNVTQVFV DTVGMPETYQARLQQSFPGI EVTVKAKADALYPVVSAASI CAKVARDQAVKKWQFVEKLQ DLDTDYGSGYPNDPKTKAWL KEHVEPVFGFPQFVRFSWR
Function Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.
Protein Name Ribonuclease H2 Subunit A
Rnase H2 Subunit A
Aicardi-Goutieres Syndrome 4 Protein
Ags4
Rnase H(35
Ribonuclease Hi Large Subunit
Rnase Hi Large Subunit
Ribonuclease Hi Subunit A
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Ribonuclease H2 Subunit A antibody
Anti-Rnase H2 Subunit A antibody
Anti-Aicardi-Goutieres Syndrome 4 Protein antibody
Anti-Ags4 antibody
Anti-Rnase H(35 antibody
Anti-Ribonuclease Hi Large Subunit antibody
Anti-Rnase Hi Large Subunit antibody
Anti-Ribonuclease Hi Subunit A antibody
Anti-RNASEH2A antibody
Anti-RNASEHI antibody
Anti-RNHIA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance