| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Telomere-associated protein RIF1 (1428-1478) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:1000-2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RIF1 |
| Gene ID: |
55183 |
| Uniprot ID: |
RIF1_HUMAN |
| Immunogen Region: |
1428-1478 |
| Specificity: |
This antibody detects endogenous levels of human RIF1 |
| Immunogen: |
Synthesized peptide derived from human RIF1 AA range: 1428-1478 |
| Function | Key regulator of TP53BP1 that plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage: acts by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs. In response to DNA damage, interacts with ATM-phosphorylated TP53BP1. Interaction with TP53BP1 leads to dissociate the interaction between NUDT16L1/TIRR and TP53BP1, thereby unmasking the tandem Tudor-like domain of TP53BP1 and allowing recruitment to DNA DSBs. Once recruited to DSBs, RIF1 and TP53BP1 act by promoting NHEJ-mediated repair of DSBs. In the same time, RIF1 and TP53BP1 specifically counteract the function of BRCA1 by blocking DSBs resection via homologous recombination (HR) during G1 phase. Also required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs. Promotes NHEJ of dysfunctional telomeres. |
| Protein Name | Telomere-Associated Protein Rif1Rap1-Interacting Factor 1 Homolog |
| Database Links | Reactome: R-HSA-5693571 |
| Cellular Localisation | NucleusChromosomeTelomereCytoplasmCytoskeletonSpindleFollowing Interaction With Tp53bp1Recruited To Sites Of Dna DamageSuch As DsbsExhibits Atm- And Tp53bp1-Dependent Localization To Uncapped Or Aberrant Telomeres And To Dna Double Strand Breaks (Dsbs)Does Not Associate With Normal Telomere StructuresLocalizes To Microtubules Of The Midzone Of The Mitotic Spindle During AnaphaseAnd To Condensed Chromosomes In Telophase |
| Alternative Antibody Names | Anti-Telomere-Associated Protein Rif1 antibodyAnti-Rap1-Interacting Factor 1 Homolog antibodyAnti-RIF1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
