Anti-RIF1 antibody (1428-1478) (STJA0006563)

SKU:
STJA0006563

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Telomere-associated protein RIF1 (1428-1478) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:1000-2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RIF1
Gene ID: 55183
Uniprot ID: RIF1_HUMAN
Immunogen Region: 1428-1478
Specificity: This antibody detects endogenous levels of human RIF1
Immunogen: Synthesized peptide derived from human RIF1 AA range: 1428-1478
Function Key regulator of TP53BP1 that plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage: acts by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs. In response to DNA damage, interacts with ATM-phosphorylated TP53BP1. Interaction with TP53BP1 leads to dissociate the interaction between NUDT16L1/TIRR and TP53BP1, thereby unmasking the tandem Tudor-like domain of TP53BP1 and allowing recruitment to DNA DSBs. Once recruited to DSBs, RIF1 and TP53BP1 act by promoting NHEJ-mediated repair of DSBs. In the same time, RIF1 and TP53BP1 specifically counteract the function of BRCA1 by blocking DSBs resection via homologous recombination (HR) during G1 phase. Also required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs. Promotes NHEJ of dysfunctional telomeres.
Protein Name Telomere-Associated Protein Rif1
Rap1-Interacting Factor 1 Homolog
Database Links Reactome: R-HSA-5693571
Cellular Localisation Nucleus
Chromosome
Telomere
Cytoplasm
Cytoskeleton
Spindle
Following Interaction With Tp53bp1
Recruited To Sites Of Dna Damage
Such As Dsbs
Exhibits Atm- And Tp53bp1-Dependent Localization To Uncapped Or Aberrant Telomeres And To Dna Double Strand Breaks (Dsbs)
Does Not Associate With Normal Telomere Structures
Localizes To Microtubules Of The Midzone Of The Mitotic Spindle During Anaphase
And To Condensed Chromosomes In Telophase
Alternative Antibody Names Anti-Telomere-Associated Protein Rif1 antibody
Anti-Rap1-Interacting Factor 1 Homolog antibody
Anti-RIF1 antibody

Information sourced from Uniprot.org

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