Anti-RICTOR antibody (1043-1708) (STJ11100924)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RICTOR (1043-1708) antibody for use in WB and ELISA in rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RICTOR |
| Gene ID | 253260 |
| Uniprot ID | RICTR_HUMAN |
| Immunogen Region | 1043-1708 |
| Immunogen Sequence | SMFILEDDRFGSSSTSTFFL DINEDTEPTFYDRSGPIKDK NSFPFFASSKLVKNRILNSL TLPNKKHRSSSDPKGGKLSS ESKTSNRRIRTLTEPSVDFN |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1043-1142 of human RICTOR (NP_689969.2). |
Additional Info
| Post Translational Modifications | Phosphorylated by MTOR.when part of mTORC2. Phosphorylated at Thr-1135 by RPS6KB1 downstream of the mTORC1 complex: phosphorylation of RICTOR inhibits mTORC2 signaling by creating a binding site for 14-3-3 proteins. Phosphorylated at Thr-1695 by GSK3A and GSK3B which facilitates RICTOR ubiquitination and subsequent degradation. Phosphorylated at Ser-1235 by GSK3B in response to endoplasmic stress, inhibiting mTORC2 signaling. Ubiquitinated by the SCF(FBXW7) complex, leading to its degradation by the proteasome. Deubiquitinated by USP9X.deubiquitination stabilizes RICTOR and enhances its binding to MTOR, thus promoting mTORC2 complex assembly. Acetylated by EP300/p300 in response to glucose, leading to activate the mTORC2 complex. Acetylation by BLOC1S1/GCN5L1 in response to hypotoxic stress protects RICTOR against ubiquitination and subsequent degradation by the proteasome. |
| Function | Component of the mechanistic target of rapamycin complex 2 (mTORC2), which transduces signals from growth factors to pathways involved in proliferation, cytoskeletal organization, lipogenesis and anabolic output. In response to growth factors, mTORC2 phosphorylates and activates AGC protein kinase family members, including AKT (AKT1, AKT2 and AKT3), PKC (PRKCA, PRKCB and PRKCE) and SGK1. In contrast to mTORC1, mTORC2 is nutrient-insensitive. Within the mTORC2 complex, RICTOR probably acts as a molecular adapter. RICTOR is responsible for the FKBP12-rapamycin-insensitivity of mTORC2. mTORC2 plays a critical role in AKT1 activation by mediating phosphorylation of different sites depending on the context, such as 'Thr-450', 'Ser-473', 'Ser-477' or 'Thr-479', facilitating the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDPK1/PDK1 which is a prerequisite for full activation. mTORC2 catalyzes the phosphorylation of SGK1 at 'Ser-422' and of PRKCA on 'Ser-657'. The mTORC2 complex also phosphorylates various proteins involved in insulin signaling, such as FBXW8 and IGF2BP1. mTORC2 acts upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. |
| Protein Name | Rapamycin-Insensitive Companion Of MtorAvo3 HomologHavo3 |
| Database Links | Reactome: R-HSA-1257604Reactome: R-HSA-389357Reactome: R-HSA-5218920Reactome: R-HSA-5674400Reactome: R-HSA-6804757Reactome: R-HSA-9856530 |
| Cellular Localisation | Cell MembraneEndoplasmic Reticulum MembraneLysosome MembraneThe Mtorc2 Complex Localizes To Membranes: Mtorc2 Is Active At The Plasma MembraneEndoplasmic Reticulum Membrane And LysosomesIin Lysosomal MembraneMtorc2 Is Sensitive To Lysosomal Positioning In The Cell |
| Alternative Antibody Names | Anti-Rapamycin-Insensitive Companion Of Mtor antibodyAnti-Avo3 Homolog antibodyAnti-Havo3 antibodyAnti-RICTOR antibodyAnti-KIAA1999 antibody |
Information sourced from Uniprot.org