| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RICTOR (1043-1708) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RICTOR |
| Gene ID: |
253260 |
| Uniprot ID: |
RICTR_HUMAN |
| Immunogen Region: |
1043-1708 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1043-1708 of human RICTOR (NP_689969.2). |
| Immunogen Sequence: |
SMFILEDDRFGSSSTSTFFL DINEDTEPTFYDRSGPIKDK NSFPFFASSKLVKNRILNSL TLPNKKHRSSSDPKGGKLSS ESKTSNRRIRTLTEPSVDFN |
| Post Translational Modifications | Phosphorylated by MTOR.when part of mTORC2. Phosphorylated at Thr-1135 by RPS6KB1.phosphorylation of RICTOR inhibits mTORC2 and AKT1 signaling. Phosphorylated at Thr-1695 by GSK3A and GSK3B which facilitates RICTOR ubiquitination and subsequent degradation. Ubiquitinated by the SCF(FBXW7) complex, leading to its degradation by the proteasome. Deubiquitinated by USP9X.deubiquitination stabilizes RICTOR and enhances its binding to MTOR, thus promoting mTORC2 complex assembly. |
| Function | Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development. |
| Protein Name | Rapamycin-Insensitive Companion Of MtorAvo3 HomologHavo3 |
| Database Links | Reactome: R-HSA-1257604Reactome: R-HSA-389357Reactome: R-HSA-5218920Reactome: R-HSA-5674400Reactome: R-HSA-6804757 |
| Alternative Antibody Names | Anti-Rapamycin-Insensitive Companion Of Mtor antibodyAnti-Avo3 Homolog antibodyAnti-Havo3 antibodyAnti-RICTOR antibodyAnti-KIAA1999 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
