Anti-RIC8A antibody (362-531) (STJ112429)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ112429
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General Information

Short DescriptionRabbit polyclonal RIC8A (362-531) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolRIC8A
Gene ID60626
Uniprot IDRIC8A_HUMAN
Immunogen Region362-531
Immunogen SequenceDVRTRPEVGEMLRNKLVRLM THLDTDVKRVAAEFLFVLCS ESVPRFIKYTGYGNAAGLLA ARGLMAGGRPEGQYSEDEDT DTDEYKEAKASINPVTGRVE EKPPNPMEGMTEEQKEHEAM KLVTMFDKLSRNRVIQPMGM SPRGHLTSLQDAMCETMEQQ LSSDPDSDPD
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 362-531 of human RIC8A (NP_001273063.1).

Additional Info

Post Translational Modifications Phosphorylated at Ser-436 and Thr-441 by CK2, stabilizing its interface with G alpha proteins.
Function Chaperone that specifically binds and folds nascent G alpha proteins prior to G protein heterotrimer formation, promoting their stability and activity: folds GNAI1, GNAO1, GNA13 and GNAQ. Does not fold G(s) G-alpha proteins GNAS nor GNAL. Also acts as a guanine nucleotide exchange factor (GEF) for G alpha proteins by stimulating exchange of bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein (GNAI1), possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.
Protein Name Chaperone Ric-8a
Synembryn-A
Database Links
Cellular Localisation Cytoplasm
Cell Cortex
Alternative Antibody Names Anti-Chaperone Ric-8a antibody
Anti-Synembryn-A antibody
Anti-RIC8A antibody

Information sourced from Uniprot.org

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