| Short Description : | Rabbit polyclonal RIC8A (362-531) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RIC8A |
| Gene ID: | 60626 |
| Uniprot ID: | RIC8A_HUMAN |
| Immunogen Region: | 362-531 |
| Immunogen Sequence: | DVRTRPEVGEMLRNKLVRLM THLDTDVKRVAAEFLFVLCS ESVPRFIKYTGYGNAAGLLA ARGLMAGGRPEGQYSEDEDT DTDEYKEAKASINPVTGRVE EKPPNPMEGMTEEQKEHEAM KLVTMFDKLSRNRVIQPMGM SPRGHLTSLQDAMCETMEQQ LSSDPDSDPD |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 362-531 of human RIC8A (NP_001273063.1). |
| Post Translational Modifications | Phosphorylated at Ser-436 and Thr-441 by CK2, stabilizing its interface with G alpha proteins. |
| Function | Chaperone that specifically binds and folds nascent G alpha proteins prior to G protein heterotrimer formation, promoting their stability and activity: folds GNAI1, GNAO1, GNA13 and GNAQ. Does not fold G(s) G-alpha proteins GNAS nor GNAL. Also acts as a guanine nucleotide exchange factor (GEF) for G alpha proteins by stimulating exchange of bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein (GNAI1), possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation. |
| Protein Name | Chaperone Ric-8aSynembryn-A |
| Database Links | |
| Cellular Localisation | CytoplasmCell Cortex |
| Alternative Antibody Names | Anti-Chaperone Ric-8a antibodyAnti-Synembryn-A antibodyAnti-RIC8A antibody |
Information sourced from Uniprot.org
