Biotinylated STJ71217 (0. 3ยตg/ml) staining of HepG2 lysate (35ยตg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-ribosomal protein L8, Biotinylated antibody (Internal) {Biotin} (STJ73212)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationBiotin
IsotypeIgG
STJ73212-100
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General Information

Short DescriptionGoat polyclonal antibody anti-ribosomal protein L8, Biotinylated (Internal) is suitable for use in ELISA and Western Blot research applications.
ApplicationsPep-ELISA/WB
HostGoat
ReactivityHuman/Mouse/Rat/Dog
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationBiotin
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:128000.
WB: Approx 28kDa band observed in lysates of cell line HepG2 (calculated MW of 28.0kDa according to NP_000964.1). See non-biotinylated parental product's datasheet for further QC data. Re
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20ยฐC on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolRPL8
Gene ID6132
Uniprot IDRL8_HUMAN
Accession NumberNP_000964.1
Immunogen RegionInternal
Immunogen SequenceKAGRAYHKYKAKRNC.
SpecificityReported variants represent identical protein: NP_000964.1, NP_150644.1, NP_001304700.1, NP_001304711.1

Additional Info

Function Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.
Protein Name Large Ribosomal Subunit Protein Ul2
60s Ribosomal Protein L8
Database Links Reactome: R-HSA-156827
Reactome: R-HSA-156902
Reactome: R-HSA-1799339
Reactome: R-HSA-192823
Reactome: R-HSA-2408557
Reactome: R-HSA-6791226
Reactome: R-HSA-72689
Reactome: R-HSA-72706
Reactome: R-HSA-72764
Reactome: R-HSA-9010553
Reactome: R-HSA-9629569
Reactome: R-HSA-9633012
Reactome: R-HSA-975956
Reactome: R-HSA-975957
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Large Ribosomal Subunit Protein Ul2 antibody
Anti-60s Ribosomal Protein L8 antibody
Anti-RPL8 antibody

Information sourced from Uniprot.org

Citations

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