Anti-RheB-Y35N Mutant antibody (STJA0033664)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from the internal region of RHEB which includes the mutation of Y35N, human origin.
General Information
| Short Description | Mouse monoclonal anti-RheB-Y35N Mutant for use in WB, ICC, IF and ELISA in Vertebrates samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ICC/IF/ELISA |
| Host | Mouse |
| Reactivity | Vertebrates |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Purified from ascites |
| Dilution Range | WB-1:500-1:1000IHC-P-1:50-1:100ELISA-1:1000-1:2000IF-1:50-1:200 |
| Formulation | Liquid in PBS (without Mg2+ and Ca2+) , pH 7.4, 150 mM NaCl, 50% glycerol |
| Storage Instruction | Store at-20°C. Avoid freeze/thaw cycles. |
Target Information
| Immunogen | A synthetic peptide from the internal region of RHEB which includes the mutation of Y35N, human origin. |
Additional Info
| Background | RheB (Ras Homolog Enriched in Brain) is an evolutionarily conserved member of the Ras-family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, RheB is widely expressed in other tissues and may be induced by growth factor stimulation. RheB has an important role in regulating the insulin/TOR signaling pathway. The mammalian target of rapamycin, mTOR, is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with translation and cell growth. The tuberin/hamartin (TSC2/TSC1) complex inhibits mTOR activity indirectly by inhibiting RheB via tuberin s GAP activity. |
Information sourced from Uniprot.org