• Immunofluorescence analysis of U-2 OS cells using RGS4 Rabbit polyclonal antibody (STJ119881) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using RGS4 Rabbit polyclonal antibody (STJ119881) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using RGS4 Rabbit polyclonal antibody (STJ119881) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using RGS4 Rabbit polyclonal antibody (STJ119881) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-RGS4 antibody (1-100) (STJ119881)

SKU:
STJ119881

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RGS4 (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RGS4
Gene ID: 5999
Uniprot ID: RGS4_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RGS4 (NP_005604.1).
Immunogen Sequence: MCKGLAGLPASCLRSAKDMK HRLGFLLQKSDSCEHNSSHN KKDKVVICQRVSQEEVKKWA ESLENLISHECGLAAFKAFL KSEYSEENIDFWISCEEYKK
Tissue Specificity Expressed in brain and heart. Expressed in brain at protein level. Expressed in prefontal and visual cortex. Isoform 4 and isoform 5 are expressed ubiquitously. Isoform 1, isoform 2 and isoform 3 are not expressed in the cerebellum.
Post Translational Modifications Palmitoylated on Cys-2 and/or Cys-12. Phosphorylated by cyclic GMP-dependent protein kinase.
Function Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Activity on G(z)-alpha is inhibited by phosphorylation of the G-protein. Activity on G(z)-alpha and G(i)-alpha-1 is inhibited by palmitoylation of the G-protein.
Protein Name Regulator Of G-Protein Signaling 4
Rgp4
Rgs4
Database Links Reactome: R-HSA-416476
Reactome: R-HSA-418594
Reactome: R-HSA-418597
Alternative Antibody Names Anti-Regulator Of G-Protein Signaling 4 antibody
Anti-Rgp4 antibody
Anti-Rgs4 antibody
Anti-RGS4 antibody

Information sourced from Uniprot.org

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