Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using RGL3 Rabbit polyclonal antibody (STJ11104874) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RGL3 antibody (1-106 aa) (STJ11104874)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11104874
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General Information

Short DescriptionRabbit polyclonal RGL3 (1-106 aa) antibody for use in WB and ELISA in a.thaliana samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityA.thaliana
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:100-1:500
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Immunogen Region1-106 aa
Immunogen SequenceMKRSHQETSVEEEAPSMVEK LENGCGGGGDDNMDEFLAVL GYKVRSSDMADVAQKLEQLE MVLSNDIASSSNAFNDTVHY NPSDLSGWAQSMLSDLNYYP DLDPNR
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 1-106 of Arabidopsis thaliana RGL3. (NP_197251.1).

Additional Info

Background Encodes a DELLA subfamily member that acts as a negative regulator of GA signaling and as a coactivator of ABI3 to promote seed storage protein biosynthesis during the seed maturation stage. Involved in hyperosmotic salinity response, jasmonic acid mediated signaling pathway, negative regulation of gibberellic acid mediated signaling pathway, negative regulation of seed germination, regulation of DNA-templated transcription, regulation of reactive oxygen species metabolic process, regulation of seed dormancy process, response to abscisic acid, response to ethylene, salicylic acid mediated signaling pathway.

Information sourced from Uniprot.org

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