| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Mouse/Rat/Pig/Dog/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-RFX5 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:1000.WB: Approx 70kDa band observed in lysates of cell line Daudi and in NIH3T3 nuclear lysates (calculated MW of 65.3kDa according to NP_000440.1). Recommended concentration: 1-3µg/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | RFX5 |
| Gene ID: | 5993 |
| Uniprot ID: | RFX5_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_000440.1 |
| Specificity: | Reported variants represent identical protein: NP_000440.1, NP_001020774.1 |
| Immunogen Sequence: | TGDKSSEPSTLSNE |
| Post Translational Modifications | Phosphorylated. |
| Function | Activates transcription from class II MHC promoters. Recognizes X-boxes. Mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters. |
| Protein Name | Dna-Binding Protein Rfx5Regulatory Factor X 5 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Dna-Binding Protein Rfx5 antibodyAnti-Regulatory Factor X 5 antibodyAnti-RFX5 antibody |
Information sourced from Uniprot.org