| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Gene Symbol: |
RECQL5 |
| Gene ID: |
9400 |
| Uniprot ID: |
RECQ5_HUMAN |
| Immunogen Region: |
400-500 aa |
| Specificity: |
A synthetic peptide corresponding to a sequence within amino acids 400-500 of human RECQL5 (NP_004250.4). |
| Immunogen Sequence: |
DALVTFCEELGCRHAAIAKY FGDALPACAKGCDHCQNPTA VRRRLEALERSSSWSKTCIG PSQGNGFDPELYEGGRKGYG DFSRYDEGSGGSGDEGRDEA H |
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Phosphorylated by CDK1 at Ser-727.this phosphorylation is required for RECQL5-mediated disruption of RAD51 filaments on stalled replication forks. |
| Function | DNA helicase that plays an important role in DNA replication, transcription and repair. Probably unwinds DNA in a 3'-5' direction (Probable). Binds to the RNA polymerase II subunit POLR2A during transcription elongation and suppresses transcription-associated genomic instability. Associates also with POLR1A and enforces the stability of ribosomal DNA arrays. Plays an important role in mitotic chromosome separation after cross-over events and cell cycle progress. Mechanistically, removes RAD51 filaments protecting stalled replication forks at common fragile sites and stimulates MUS81-EME1 endonuclease leading to mitotic DNA synthesis. Required for efficient DNA repair, including repair of inter-strand cross-links. Stimulates DNA decatenation mediated by TOP2A. Prevents sister chromatid exchange and homologous recombination. A core helicase fragment (residues 11-609) binds preferentially to splayed duplex, looped and ssDNA. |
| Protein Name | Atp-Dependent Dna Helicase Q5Dna 3'-5' Helicase Recq5Dna Helicase - Recq-Like Type 5Recq5Recq Protein-Like 5 |
| Cellular Localisation | Isoform Beta: NucleusNucleoplasmNucleusRecruited To Sites Of Dna DamageSuch As Single-Strand Breaks And Inter-Strand Cross-LinksAnd At Stalled Replication ForksRe-Localizes From The Nucleolus Into The Nucleus After Replicative Stress And Significantly Associates With Wrn During S-PhaseIsoform Alpha: CytoplasmIsoform Gamma: Cytoplasm |
| Alternative Antibody Names | Anti-Atp-Dependent Dna Helicase Q5 antibodyAnti-Dna 3'-5' Helicase Recq5 antibodyAnti-Dna Helicase - Recq-Like Type 5 antibodyAnti-Recq5 antibodyAnti-Recq Protein-Like 5 antibodyAnti-RECQL5 antibodyAnti-RECQ5 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
