Anti-Recombinant-VGlut1 antibody (C-Term) [N28/9] {FL650 Ex: 655 nm, Em: 676 nm} (STJA0040421)
SPECIFICATIONS
ClonalityMonoclonal
HostChicken
ConjugationFL650 Ex: 655 nm, Em: 676 nm
IsotypeIgY
ImmunogenFusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1 (accession number Q62634) produced recombinantly in E. Coli
General Information
| Short Description | Chicken monoclonal anti-Recombinant-VGlut1 (C-Term) for use in IHC, ICC and FC in Fish, Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/ICC/FC |
| Host | Chicken |
| Reactivity | Fish/Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | N28/9 |
| Isotype | IgY |
| Conjugation | FL650 Ex: 655 nm, Em: 676 nm |
| Concentration | 0.5 mg/mL |
| Purification | Purified by affinity chromatography |
| Dilution Range | IHC 1:100-1:200ICC 1:100-1:200 |
| Formulation | 1X PBS, 0.05% Sodium Azide pH 7.4 |
| Storage Instruction | Aliquot and store at โค-20ยฐC for long term storage. For short term storage, store at 2-8ยฐC. For maximum recovery of product, centrifuge the vial prior to removing the cap. |
Target Information
| Gene Symbol | Slc17a7 |
| Gene ID | 116638 |
| Uniprot ID | VGLU1_RAT |
| Immunogen | Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1 (accession number Q62634) produced recombinantly in E. Coli |
| Immunogen Region | C-Term |
| Immunogen Sequence | Rat |
| Specificity | Does not cross-react with VGlut2 or VGlut3 |
Additional Info
| Function | Multifunctional transporter that transports L-glutamate as well as multiple ions such as chloride, proton, potassium, sodium and phosphate. At the synaptic vesicle membrane, mainly functions as an uniporter which transports preferentially L-glutamate but also phosphate from the cytoplasm into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. The L-glutamate or phosphate uniporter activity is electrogenic and is driven by the proton electrochemical gradient, mainly by the electrical gradient established by the vacuolar H(+)-ATPase across the synaptic vesicle membrane. In addition, functions as a chloride channel that allows a chloride permeation through the synaptic vesicle membrane that affects the proton electrochemical gradient and promotes synaptic vesicles acidification. Moreover, may function as a K(+)/H(+) antiport allowing to maintain the electrical gradient and to decrease chemical gradient and therefore sustain vesicular glutamate uptake. The vesicular K(+)/H(+) antiport activity is electroneutral. At the plasma membrane, following exocytosis, functions as a symporter of Na(+) and phosphate from the extracellular space to the cytoplasm allowing synaptic phosphate homeostasis regulation. The symporter activity is driven by an inside negative membrane potential and is electrogenic. Is necessary for synaptic signaling of visual-evoked responses from photoreceptors. |
| Protein Name | Vesicular Glutamate Transporter 1Vglut1Brain-Specific Na(+-Dependent Inorganic Phosphate CotransporterSolute Carrier Family 17 Member 7 |
| Database Links | Reactome: R-RNO-210500Reactome: -RNO-428643 |
| Cellular Localisation | Cytoplasmic VesicleSecretory VesicleSynaptic Vesicle MembraneCell MembraneMulti-Pass Membrane ProteinSynapseSynaptosome |
| Alternative Antibody Names | Anti-Vesicular Glutamate Transporter 1 antibodyAnti-Vglut1 antibodyAnti-Brain-Specific Na(+-Dependent Inorganic Phosphate Cotransporter antibodyAnti-Solute Carrier Family 17 Member 7 antibodyAnti-Slc17a7 antibodyAnti-Bnpi antibodyAnti-Vglut1 antibody |
Information sourced from Uniprot.org