Anti-Recombinant-VAMP8 antibody [RM2M98] (STJA0026161)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal anti-Recombinant-Vesicle-Associated Membrane Protein 8 for use in FC, IF, IHC, IP and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC/IF/IHC/IP/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | RM2M98 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Protein A/G purified from cell culture supernatant |
| Dilution Range | FCM: 1:20-1:100, IF: 1:50-1:200, IHC: 1:100-1:200, IP: 1:20-1:50, WB: 1:1000-1:2000 |
| Formulation | 0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium Azide |
| Storage Instruction | Suitable for storage at +4°C between 1-2 weeks. For longer term store at-20°C for up to 12 months. |
Target Information
| Gene Symbol | VAMP8 |
| Gene ID | 8673 |
| Uniprot ID | VAMP8_HUMAN |
Additional Info
| Post Translational Modifications | (Microbial infection) Stearoylated By S.flexneri N-epsilon-fatty acyltransferase IcsB, thereby disrupting the host actin cytoskeleton. |
| Function | SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex. Also required for dense-granule secretion in platelets. Also plays a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes. Also participates in the activation of type I interferon antiviral response through a TRIM6-dependent mechanism. |
| Protein Name | Vesicle-Associated Membrane Protein 8Vamp-8EndobrevinEdb |
| Database Links | Reactome: R-HSA-1236974Reactome: R-HSA-199992Reactome: R-HSA-432720Reactome: R-HSA-432722Reactome: R-HSA-6798695Reactome: R-HSA-8856825Reactome: R-HSA-8856828 |
| Cellular Localisation | Lysosome MembraneSingle-Pass Type Iv Membrane ProteinEarly Endosome MembraneLate Endosome MembraneCell MembraneZymogen Granule MembranePerinuclear Vesicular Structures Of The Early And Late EndosomesCoated PitsAnd Trans-GolgiSub-Tight Junctional Domain In Retinal Pigment Epithelium CellsMidbody Region During CytokinesisLumenal OrientedApical Membranes Of Nephric Tubular CellCycles Through The Apical But Not Through The Basolateral Plasma MembraneApical Region Of Acinar CellsIn Zymogen Granule Membranes |
| Alternative Antibody Names | Anti-Vesicle-Associated Membrane Protein 8 antibodyAnti-Vamp-8 antibodyAnti-Endobrevin antibodyAnti-Edb antibodyAnti-VAMP8 antibody |
Information sourced from Uniprot.org