Anti-Recombinant-Tyrosine Hydroxylase antibody [LNC1] {FL490 Ex: 491 nm, Em: 515 nm} (STJA0040572)
SPECIFICATIONS
ClonalityMonoclonal
HostChicken
ConjugationFL490 Ex: 491 nm, Em: 515 nm
IsotypeIgY
ImmunogenTyrosine Hydroxylase purified from PC12 cells
General Information
| Short Description | Chicken monoclonal anti-Recombinant-Tyrosine Hydroxylase for use in ICC and IHC in Chicken, Frog, Human, Lizard, Monkey, Mouse, Rat, Vole and Zebrafish samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ICC/IHC |
| Host | Chicken |
| Reactivity | Chicken/Frog/Human/Lizard/Monkey/Mouse/Rat/Vole/Zebrafish |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | LNC1 |
| Isotype | IgY |
| Conjugation | FL490 Ex: 491 nm, Em: 515 nm |
| Concentration | 0.5 mg/mL |
| Purification | Purified by affinity chromatography |
| Formulation | 1X PBS, 0.05% Sodium Azide pH 7.4 |
| Storage Instruction | Aliquot and store at ≤-20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap. |
Target Information
| Gene Symbol | TH |
| Gene ID | 7054 |
| Uniprot ID | TY3H_HUMAN |
| Immunogen | Tyrosine Hydroxylase purified from PC12 cells |
| Immunogen Sequence | Human |
| Specificity | Recognizes an epitope outside of the regulatory N-terminus. Recognizes a protein of approximately 59-61 kDa by Western blot. Does not react with the following on Western Blots: purified dopamine-beta-hydroxylase, phenylalanine hydroxylase, trytophan |
Additional Info
| Tissue Specificity | Mainly expressed in the brain and adrenal glands. |
| Post Translational Modifications | Phosphorylated on Ser-19, Ser-62 and Ser-71 by several protein kinases with different site specificities. Phosphorylation at Ser-62 and Ser-71 leads to an increase of TH activity. Phosphorylation at Ser-71 activates the enzyme and also counteracts the feedback inhibition of TH by catecholamines. Phosphorylation of Ser-19 and Ser-62 triggers the proteasomal degradation of TH through the ubiquitin-proteasome pathway. Phosphorylation at Ser-62 facilitates transport of TH from the soma to the nerve terminals via the microtubule network. Phosphorylation at Ser-19 induces the high-affinity binding to the 14-3-3 protein YWHAG.this interaction may influence the phosphorylation and dephosphorylation of other sites. Ser-19 increases the phosphorylation at Ser-71 in a hierarchical manner, leading to increased activity. |
| Function | Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of catecholamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa. In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan with lower specificity. Positively regulates the regression of retinal hyaloid vessels during postnatal development. Isoform 5: Lacks catalytic activity. Isoform 6: Lacks catalytic activity. |
| Protein Name | Tyrosine 3-MonooxygenaseTyrosine 3-HydroxylaseTh |
| Database Links | Reactome: R-HSA-209905 |
| Cellular Localisation | CytoplasmPerinuclear RegionNucleusCell ProjectionAxonCytoplasmic VesicleSecretory VesicleSynaptic VesicleWhen Phosphorylated At Ser-19 Shows A Nuclear Distribution And When Phosphorylated At Ser-31 As Well At Ser-40 Shows A Cytosolic DistributionExpressed In Dopaminergic Axons And Axon Terminals |
| Alternative Antibody Names | Anti-Tyrosine 3-Monooxygenase antibodyAnti-Tyrosine 3-Hydroxylase antibodyAnti-Th antibodyAnti-TH antibodyAnti-TYH antibody |
Information sourced from Uniprot.org