| Host: |
Mouse |
| Applications: |
IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Recombinant-TLR4 is suitable for use in Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
SM0617 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1 |
| Formulation: |
PBS with 0.15% ProClin300, 100 Mu g/mL BSA and 50% glycerol. |
| Purification: |
Affinity purification |
| Dilution Range: |
IHC/IF (H, M, R) 1:500-1:1000 |
| Storage Instruction: |
Store at-20C for up to one year, and avoid repeated freeze-thaw cycles. |
| Gene Symbol: |
Tlr4 |
| Gene ID: |
21898 |
| Uniprot ID: |
TLR4_MOUSE |
| Immunogen: |
Recombinant protein corresponding to Mouse TLR4 |
| Post Translational Modifications | Phosphorylated on tyrosine residues by LYN after binding lipopolysaccharide. |
| Function | Transmembrane receptor that functions as a pattern recognition receptor recognizing pathogen- and damage-associated molecular patterns (PAMPs and DAMPs) to induce innate immune responses via downstream signaling pathways. At the plasma membrane, cooperates with LY96 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni(2+). Mechanistically, acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Alternatively, CD14-mediated TLR4 internalization via endocytosis is associated with the initiation of a MYD88-independent signaling via the TICAM1-TBK1-IRF3 axis leading to type I interferon production. In addition to the secretion of proinflammatory cytokines, initiates the activation of NLRP3 inflammasome and formation of a positive feedback loop between autophagy and NF-kappa-B signaling cascade. In complex with TLR6, promotes inflammation in monocytes/macrophages by associating with TLR6 and the receptor CD86. Upon ligand binding, such as oxLDL or amyloid-beta 42, the TLR4:TLR6 complex is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway. In myeloid dendritic cells, vesicular stomatitis virus glycoprotein G but not LPS promotes the activation of IRF7, leading to type I IFN production in a CD14-dependent manner. (Microbial infection) Required for the virulence of fungus C.neoformans var. grubii serotype A (strain KN99) CPL1. Independently of Ly96/Md2, activated by CPL1 which results in a type 2 inflammation response characterized by Arg1/arginase-1 induction in interstitial macrophages. (Microbial infection) Mediates activation of bone marrow-derived dendritic cells and macrophages, and production of pro-inflammatory cytokines, such as IL12 (IL12B/IL12A), triggered by Toxoplasma gondii micronemal protein 4 (MIC4) and micronemal protein 1 (MIC1). Mediates production of anti-inflammatory cytokine IL10 triggered by Toxoplasma gondii micronemal protein 4 (MIC4) and micronemal protein 1 (MIC1) in murine bone marrow-derived macrophages. |
| Protein Name | Toll-Like Receptor 4Cd Antigen Cd284 |
| Database Links | Reactome: R-MMU-140534Reactome: -MMU-166016Reactome: -MMU-166166Reactome: -MMU-2562578Reactome: -MMU-5686938Reactome: -MMU-936964Reactome: -MMU-937041Reactome: -MMU-937072Reactome: -MMU-9707616Reactome: -MMU-975163Reactome: -MMU-9824878 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinEarly EndosomeCell ProjectionRuffleUpon Complex Formation With Cd36 And Tlr6Internalized Through Dynamin-Dependent EndocytosisColocalizes With Rftn1 At Cell Membrane And Then Together With Rftn1 Moves To EndosomesUpon Lipopolysaccharide Stimulation(Microbial Infection) Endocytosed Upon Interaction With Fungus CNeoformans VarGrubii Serotype A (Strain Kn99) Cpl1 At The Macrophage Cell Surface |
| Alternative Antibody Names | Anti-Toll-Like Receptor 4 antibodyAnti-Cd Antigen Cd284 antibodyAnti-Tlr4 antibodyAnti-Lps antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
