Anti-TLR4 antibody [HTA125] (STJ16100496)

SKU:
STJ16100496

Current Stock:
Host: Mouse
Applications: FC, ELISA, IF, IP, WB
Reactivity: Human, Canine, Monkey
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Mouse monoclonal antibody anti-TLR4 is suitable for use in Flow Cytometry, ELISA, Immunofluorescence, Immunoprecipitation and Western Blot research applications.
Clonality: Monoclonal
Clone ID: HTA125
Conjugation: Unconjugated
Isotype: IgG2a
Formulation: PBS with 0.1% BSA and 0.02% sodium azide
Storage Instruction: Store at 2-8°C upon receipt.
Gene Symbol: TLR4
Gene ID: 7099
Uniprot ID: TLR4_HUMAN
Tissue Specificity Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
Post Translational Modifications N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS. Phosphorylated on tyrosine residues by LYN after binding lipopolysaccharide.
Function Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni(2+). Responses triggered by Ni(2+) require non-conserved histidines and are, therefore, species-specific. Both M.tuberculosis HSP70 (dnaK) and HSP65 (groEL-2) act via this protein to stimulate NF-kappa-B expression. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-). Stimulation of monocytes in vitro with M.tuberculosis PstS1 induces p38 MAPK and ERK1/2 activation primarily via TLR2, but also partially via this receptor. Activated by the signaling pathway regulator NMI which acts as damage-associated molecular patterns (DAMPs) in response to cell injury or pathogen invasion, therefore promoting nuclear factor NF-kappa-B activation.
Protein Name Toll-Like Receptor 4
Htoll
Cd Antigen Cd284
Database Links Reactome: R-HSA-1236974
Reactome: R-HSA-140534
Reactome: R-HSA-166016
Reactome: R-HSA-166058
Reactome: R-HSA-166166
Reactome: R-HSA-2562578
Reactome: R-HSA-5602498
Reactome: R-HSA-5603041
Reactome: R-HSA-5686938
Reactome: R-HSA-936964
Reactome: R-HSA-937041
Reactome: R-HSA-937072
Reactome: R-HSA-9707616
Reactome: R-HSA-975163
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Early Endosome
Cell Projection
Ruffle
Upon Complex Formation With Cd36 And Tlr6
Internalized Through Dynamin-Dependent Endocytosis
Colocalizes With Rftn1 At Cell Membrane And Then Together With Rftn1 Moves To Endosomes
Upon Lipopolysaccharide Stimulation
Alternative Antibody Names Anti-Toll-Like Receptor 4 antibody
Anti-Htoll antibody
Anti-Cd Antigen Cd284 antibody
Anti-TLR4 antibody

Information sourced from Uniprot.org

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