||Mouse monoclonal anti-TLR4 antibody is suitable for use in NA.
||FOR RESEARCH USE ONLY (RUO).
| Post Translational Modifications || N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS. Phosphorylated on tyrosine residues by LYN after binding lipopolysaccharide. |
| Function || Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni(2+). Responses triggered by Ni(2+) require non-conserved histidines and are, therefore, species-specific. Both M.tuberculosis HSP70 (dnaK) and HSP65 (groEL-2) act via this protein to stimulate NF-kappa-B expression. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-). Stimulation of monocytes in vitro with M.tuberculosis PstS1 induces p38 MAPK and ERK1/2 activation primarily via TLR2, but also partially via this receptor. |
| Protein Name || Toll-Like Receptor 4HtollCd Antigen Cd284 |
| Database Links || Reactome: R-HSA-1236974Reactome: R-HSA-140534Reactome: R-HSA-166016Reactome: R-HSA-166058Reactome: R-HSA-166166Reactome: R-HSA-2562578Reactome: R-HSA-5602498Reactome: R-HSA-5603041Reactome: R-HSA-5686938Reactome: R-HSA-936964Reactome: R-HSA-937041Reactome: R-HSA-937072Reactome: R-HSA-975163 |
| Cellular Localisation || Cell MembraneSingle-Pass Type I Membrane ProteinEarly EndosomeCell ProjectionRuffleUpon Complex Formation With Cd36 And Tlr6Internalized Through Dynamin-Dependent EndocytosisColocalizes With Rftn1 At Cell Membrane And Then Together With Rftn1 Moves To EndosomesUpon Lipopolysaccharide Stimulation |
| Alternative Antibody Names || Anti-Toll-Like Receptor 4 antibodyAnti-Htoll antibodyAnti-Cd Antigen Cd284 antibodyAnti-TLR4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance