Anti-Recombinant-PLAT/tPA antibody [RM1H43] (STJA0023334)

SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJA0023334
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General Information

Short DescriptionRabbit monoclonal anti-Recombinant-Tissue-Type Plasminogen Activator for use in IHC and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsIHC/WB
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDRM1H43
IsotypeIgG
ConjugationUnconjugated
PurificationProtein A/G purified from cell culture supernatant
Dilution RangeIHC: 1:100-1:200
Formulation0.01M PBS
Storage InstructionSuitable for storage at +4°C between 1-2 weeks. For longer term store at-20°C for up to 12 months.

Target Information

Gene SymbolPLAT
Gene ID5327
Uniprot IDTPA_HUMAN

Additional Info

Post Translational Modifications The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa. Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin. N-glycosylation of Asn-152.the bound oligomannosidic glycan is involved in the interaction with the mannose receptor. Characterization of O-linked glycan was studied in Bowes melanoma cell line.
Function Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. During oocyte activation, plays a role in cortical granule reaction in the zona reaction, which contributes to the block to polyspermy.
Protein Name Tissue-Type Plasminogen Activator
T-Pa
T-Plasminogen Activator
Tpa
Alteplase
Reteplase Cleaved Into - Tissue-Type Plasminogen Activator Chain A - Tissue-Type Plasminogen Activator Chain B
Database Links Reactome: R-HSA-186797
Reactome: R-HSA-75205
Cellular Localisation Secreted
Extracellular Space
Alternative Antibody Names Anti-Tissue-Type Plasminogen Activator antibody
Anti-T-Pa antibody
Anti-T-Plasminogen Activator antibody
Anti-Tpa antibody
Anti-Alteplase antibody
Anti-Reteplase Cleaved Into - Tissue-Type Plasminogen Activator Chain A - Tissue-Type Plasminogen Activator Chain B antibody
Anti-PLAT antibody

Information sourced from Uniprot.org

Citations

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