Anti-Recombinant-Phospho-PTPN1-Ser352 antibody [RM1Q72] (STJA0024056)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal anti-Recombinant-Phospho-Tyrosine-Protein Phosphatase Non-Receptor Type 1-Ser352 for use in IF, IP and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IF/IP/WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | RM1Q72 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Protein A/G purified from cell culture supernatant |
| Dilution Range | IF: 1:50-1:200 |
| Formulation | 0.01M PBS |
| Storage Instruction | Suitable for storage at +4°C between 1-2 weeks. For longer term store at-20°C for up to 12 months. |
Target Information
| Gene Symbol | PTPN1 |
| Gene ID | 5770 |
| Uniprot ID | PTN1_HUMAN |
Additional Info
| Post Translational Modifications | Oxidized on Cys-215.the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a sulfenamide cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction. Ser-50 is the major site of phosphorylation as compared to Ser-242 and Ser-243. Activated by phosphorylation at Ser-50. S-nitrosylation of Cys-215 inactivates the enzyme activity. Sulfhydration at Cys-215 following endoplasmic reticulum stress inactivates the enzyme activity, promoting EIF2AK3/PERK activity. |
| Function | Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK.inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET. |
| Protein Name | Tyrosine-Protein Phosphatase Non-Receptor Type 1Protein-Tyrosine Phosphatase 1bPtp-1b |
| Database Links | Reactome: R-HSA-354192Reactome: R-HSA-6807004Reactome: R-HSA-77387Reactome: R-HSA-877312Reactome: R-HSA-8849472Reactome: R-HSA-9022699Reactome: R-HSA-912694Reactome: R-HSA-982772Reactome: R-HSA-9860927 |
| Cellular Localisation | Endoplasmic Reticulum MembranePeripheral Membrane ProteinCytoplasmic SideInteracts With Epha3 At The Cell Membrane |
| Alternative Antibody Names | Anti-Tyrosine-Protein Phosphatase Non-Receptor Type 1 antibodyAnti-Protein-Tyrosine Phosphatase 1b antibodyAnti-Ptp-1b antibodyAnti-PTPN1 antibodyAnti-PTP1B antibody |
Information sourced from Uniprot.org