| Host: |
Mouse |
| Applications: |
ELISA/IF/IHC-C |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Recombinant-MANF is suitable for use in ELISA, Immunofluorescence and Immunohistochemistry-C research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
B8 |
| Conjugation: |
Unconjugated |
| Isotype: |
mouse IgG2a |
| Formulation: |
PBS pH 7.4, with 0.1% sodium azide |
| Purification: |
MabSelect affinity chromatography |
| Dilution Range: |
ELISA 0.02 to 0.1 µg/ml; IF 1 to 20 µg/ml; IHC-C 5 µg/ml. Conformational antibody, not suitable for Western Blot application. Monoclonal antibody working titer has to be established practically for each particular antigen and assay format |
| Storage Instruction: |
Store at-20 to-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles |
| Gene Symbol: |
MANF |
| Gene ID: |
7873 |
| Uniprot ID: |
MANF_HUMAN |
| Specificity: |
Human MANF |
| Immunogen: |
Recombinant human MANF protein produced using CHO-based proprietary suspension cell line. Immunogen is purified from cell culture supernatant. Human MANF |
| Post Translational Modifications | May contain sialic acid residues. |
| Function | Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. Retained in the ER/sarcoplasmic reticulum (SR) through association with the endoplasmic reticulum chaperone protein HSPA5 under normal conditions. Up-regulated and secreted by the ER/SR in response to ER stress and hypoxia. Following secretion by the ER/SR, directly binds to 3-O-sulfogalactosylceramide, a lipid sulfatide in the outer cell membrane of target cells. Sulfatide binding promotes its cellular uptake by endocytosis, and is required for its role in alleviating ER stress and cell toxicity under hypoxic and ER stress conditions. |
| Protein Name | Mesencephalic Astrocyte-Derived Neurotrophic FactorArginine-Rich ProteinProtein Armet |
| Database Links | Reactome: R-HSA-114608 |
| Cellular Localisation | SecretedEndoplasmic Reticulum LumenSarcoplasmic Reticulum LumenRetained In The Endoplasmic Reticulum (Er)And Sarcoplasmic Reticulum (Sr) Under Normal ConditionsUp-Regulated And Secreted By The Er/Sr In Response To Er Stress And Hypoxia |
| Alternative Antibody Names | Anti-Mesencephalic Astrocyte-Derived Neurotrophic Factor antibodyAnti-Arginine-Rich Protein antibodyAnti-Protein Armet antibodyAnti-MANF antibodyAnti-ARMET antibodyAnti-ARP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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