Anti-Recombinant-IRS2 antibody [RM2L12] (STJA0025976)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal anti-Recombinant-Insulin Receptor Substrate 2 for use in FC, IF, IHC and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC/IF/IHC/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | RM2L12 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Protein A/G purified from cell culture supernatant |
| Dilution Range | FCM: 1:20-1:100, IF: 1:50-1:200, IHC: 1:100-1:200, WB: 1:1000-1:2000 |
| Formulation | 0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium Azide |
| Storage Instruction | Suitable for storage at +4°C between 1-2 weeks. For longer term store at-20°C for up to 12 months. |
Target Information
| Gene Symbol | IRS2 |
| Gene ID | 8660 |
| Uniprot ID | IRS2_HUMAN |
Additional Info
| Function | Signaling adapter protein that participates in the signal transduction from two prominent receptor tyrosine kinases, insulin receptor/INSR and insulin-like growth factor I receptor/IGF1R. Plays therefore an important role in development, growth, glucose homeostasis as well as lipid metabolism. Upon phosphorylation by the insulin receptor, functions as a signaling scaffold that propagates insulin action through binding to SH2 domain-containing proteins including the p85 regulatory subunit of PI3K, NCK1, NCK2, GRB2 or SHP2. Recruitment of GRB2 leads to the activation of the guanine nucleotide exchange factor SOS1 which in turn triggers the Ras/Raf/MEK/MAPK signaling cascade. Activation of the PI3K/AKT pathway is responsible for most of insulin metabolic effects in the cell, and the Ras/Raf/MEK/MAPK is involved in the regulation of gene expression and in cooperation with the PI3K pathway regulates cell growth and differentiation. Acts a positive regulator of the Wnt/beta-catenin signaling pathway through suppression of DVL2 autophagy-mediated degradation leading to cell proliferation. Plays a role in cell cycle progression by promoting a robust spindle assembly checkpoint (SAC) during M-phase. In macrophages, IL4-induced tyrosine phosphorylation of IRS2 leads to the recruitment and activation of phosphoinositide 3-kinase (PI3K). |
| Protein Name | Insulin Receptor Substrate 2Irs-2 |
| Database Links | Reactome: R-HSA-109704Reactome: R-HSA-112399Reactome: R-HSA-112412Reactome: R-HSA-1257604Reactome: R-HSA-1266695Reactome: R-HSA-198203Reactome: R-HSA-2219530Reactome: R-HSA-2428928Reactome: R-HSA-2586552Reactome: R-HSA-5673001Reactome: R-HSA-6811558Reactome: R-HSA-74713Reactome: R-HSA-74749Reactome: R-HSA-8853659Reactome: R-HSA-9006335Reactome: R-HSA-9027276Reactome: R-HSA-9027277Reactome: R-HSA-9027283Reactome: R-HSA-9027284Reactome: R-HSA-982772 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-Insulin Receptor Substrate 2 antibodyAnti-Irs-2 antibodyAnti-IRS2 antibody |
Information sourced from Uniprot.org