| Host: |
Mouse |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Recombinant-EIF2S1 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
SM0684 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1 |
| Formulation: |
PBS with 0.15% ProClin300, 100 Mu g/mL BSA and 50% glycerol. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB (H, M, R) 1:500-1:1000IHC/IF (M, R) 1:2000-1:8000 |
| Storage Instruction: |
Store at-20C for up to one year, and avoid repeated freeze-thaw cycles. |
| Gene Symbol: |
Eif2s1 |
| Gene ID: |
13665 |
| Uniprot ID: |
IF2A_MOUSE |
| Immunogen: |
Recombinant protein corresponding to Mouse EIF2S1 |
| Function | Member of the eIF2 complex that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex. In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF2B. EIF2S1/eIF2-alpha is a key component of the integrated stress response (ISR), required for adaptation to various stress: phosphorylation by metabolic-stress sensing protein kinases (EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2) in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to a attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activators ATF4 and QRICH1, and hence allowing ATF4- and QRICH1-mediated reprogramming. EIF2S1/eIF2-alpha also acts as an activator of mitophagy in response to mitochondrial damage: phosphorylation by EIF2AK1/HRI promotes relocalization to the mitochondrial surface, thereby triggering PRKN-independent mitophagy. |
| Protein Name | Eukaryotic Translation Initiation Factor 2 Subunit 1Eukaryotic Translation Initiation Factor 2 Subunit AlphaEif-2-AlphaEif-2aEif-2alphaEif2-Alpha |
| Database Links | Reactome: R-MMU-156827Reactome: -MMU-381042Reactome: -MMU-382556Reactome: -MMU-72649Reactome: -MMU-72695Reactome: -MMU-72702Reactome: -MMU-72706Reactome: -MMU-72731Reactome: -MMU-9840373 |
| Cellular Localisation | CytoplasmStress GranuleCytosolMitochondrionColocalizes With Nanos3 In The Stress GranulesRelocalizes To The Surface Of Mitochondria In Response To Mitochondrial Damage And Phosphorylation By Eif2ak1/Hri |
| Alternative Antibody Names | Anti-Eukaryotic Translation Initiation Factor 2 Subunit 1 antibodyAnti-Eukaryotic Translation Initiation Factor 2 Subunit Alpha antibodyAnti-Eif-2-Alpha antibodyAnti-Eif-2a antibodyAnti-Eif-2alpha antibodyAnti-Eif2-Alpha antibodyAnti-Eif2s1 antibodyAnti-Eif2a antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
