• Immunohistochemistry analysis of CD163. Sample: Rat spleen, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:600, 4C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG, 1:200, RT, 1h.

Anti-Recombinant-CD163 antibody [SR0659] (STJA0010659)

SKU:
STJA0010659

£170.50 - £233.50
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Host: Rabbit
Applications: IHC/IF
Reactivity: Human/Mouse/Rat/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Recombinant-CD163 is suitable for use in Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: SR0659
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.15% ProClin300, 100 Mu g/mL BSA and 50% glycerol.
Purification: Affinity purification
Dilution Range: IHC/IF (H, M, R, Mk) 1:300-1:600
Storage Instruction: Store at-20C for up to one year, and avoid repeated freeze-thaw cycles.
Gene Symbol: Cd163
Gene ID: 93671
Uniprot ID: C163A_MOUSE
Immunogen: Recombinant protein corresponding to Mouse CD163
Post Translational Modifications A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs.
Function Involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. After shedding, the soluble form (sCD163) may play an anti-inflammatory role.
Protein Name Scavenger Receptor Cysteine-Rich Type 1 Protein M130
Cd Antigen Cd163 Cleaved Into - Soluble Cd163
Scd163
Database Links Reactome: R-MMU-2168880
Cellular Localisation Soluble Cd163: Secreted
Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Scavenger Receptor Cysteine-Rich Type 1 Protein M130 antibody
Anti-Cd Antigen Cd163 Cleaved Into - Soluble Cd163 antibody
Anti-Scd163 antibody
Anti-Cd163 antibody
Anti-M130 antibody

Information sourced from Uniprot.org

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