| Host: | Mouse |
| Applications: | WB/IHC/IF |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal anti-Recombinant-beta III Tubulin for use in WB, IHC and IF in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Monoclonal |
| Clone ID : | SM5068 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | PBS with 0.02% sodium azide, 100 Mu g/ml BSA and 50% glycerol. |
| Purification: | Affinity purification |
| Dilution Range: | WB (H, M, R) 1:1000-1:2000IHC/IF (H, M, R, Mk) 1:600-1:1200 |
| Storage Instruction: | Store at-20C for up to one year, and avoid repeated freeze-thaw cycles. |
| Gene Symbol: | Tubb3 |
| Gene ID: | 22152 |
| Uniprot ID: | TBB3_MOUSE |
| Immunogen: | Recombinant protein corresponding to Mouse beta III Tubulin |
| Post Translational Modifications | Some glutamate residues at the C-terminus are polyglycylated, resulting in polyglycine chains on the gamma-carboxyl group. Glycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering polyglycylation levels increases polyglutamylation, and reciprocally. Cilia and flagella glycylation is required for their stability and maintenance. Flagella glycylation controls sperm motility. Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold. Glutamylation is also involved in cilia motility. Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. |
| Function | Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, alpha-beta tubulin heterodimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. TUBB3 plays a critical role in proper axon guidance and maintenance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion. Plays a role in dorsal root ganglion axon projection towards the spinal cord. |
| Protein Name | Tubulin Beta-3 Chain |
| Database Links | Reactome: R-MMU-190840Reactome: -MMU-2132295Reactome: -MMU-2467813Reactome: -MMU-2500257Reactome: -MMU-3371497Reactome: -MMU-380320Reactome: -MMU-437239Reactome: -MMU-5610787Reactome: -MMU-5617833Reactome: -MMU-5620924Reactome: -MMU-5626467Reactome: -MMU-5663220Reactome: -MMU-6807878Reactome: -MMU-6811434Reactome: -MMU-6811436Reactome: -MMU-68877Reactome: -MMU-8852276Reactome: -MMU-8955332Reactome: -MMU-9646399Reactome: -MMU-9648025Reactome: -MMU-9668328Reactome: -MMU-983189Reactome: -MMU-9833482 |
| Cellular Localisation | CytoplasmCytoskeletonCell ProjectionGrowth ConeLamellipodiumFilopodium |
| Alternative Antibody Names | Anti-Tubulin Beta-3 Chain antibodyAnti-Tubb3 antibody |
Information sourced from Uniprot.org
![Anti-Beta III Tubulin antibody [TU-20] (STJ16100477)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/149194/346815/STJ16100477_1__67885.1681998921.jpg?c=1 "Anti-Beta III Tubulin antibody [TU-20] (STJ16100477)")
