Anti-Recombinant-Bax antibody [SR0671] (STJA0010671)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenKLH conjugated Synthetic peptide corresponding to Mouse Bax
General Information
| Short Description | Rabbit monoclonal anti-Recombinant-Bax for use in IHC and IF in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SR0671 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | IHC/IF (H, M, R) 1:500-1:1000 |
| Formulation | PBS with 0.15% ProClin300, 100 Mu g/mL BSA and 50% glycerol. |
| Storage Instruction | Store at-20C for up to one year, and avoid repeated freeze-thaw cycles. |
Target Information
| Gene Symbol | BAX |
| Gene ID | 581 |
| Uniprot ID | BAX_HUMAN |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse Bax |
Additional Info
| Post Translational Modifications | Ubiquitinated in the absence of XRCC6/Ku70. Ubiquitination promotes protein degradation. Ubiquitinated on Lys-128 and Lys-190. 'Lys-63'-linked polyubiquitin chains on Lys-128 are removed by USP12. |
| Function | Plays a role in the mitochondrial apoptotic process. Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. |
| Protein Name | Apoptosis Regulator BaxBcl-2-Like Protein 4Bcl2-L-4 |
| Database Links | Reactome: R-HSA-111457Reactome: R-HSA-114294Reactome: R-HSA-5620971Reactome: R-HSA-6803204Reactome: R-HSA-6804114Reactome: R-HSA-8878166Reactome: R-HSA-9603505 |
| Cellular Localisation | Isoform Alpha: Mitochondrion Outer MembraneSingle-Pass Membrane ProteinCytoplasmNucleusColocalizes With 14-3-3 Proteins In The CytoplasmUnder Stress ConditionsUndergoes A Conformation Change That Causes Release From Jnk-Phosphorylated 14-3-3 Proteins And Translocation To The Mitochondrion MembraneUpon Sendai Virus InfectionRecruited To The Mitochondrion Through Interaction With Irf3Isoform Beta: CytoplasmIsoform Gamma: CytoplasmIsoform Delta: Cytoplasm |
| Alternative Antibody Names | Anti-Apoptosis Regulator Bax antibodyAnti-Bcl-2-Like Protein 4 antibodyAnti-Bcl2-L-4 antibodyAnti-BAX antibodyAnti-BCL2L4 antibody |
Information sourced from Uniprot.org