Anti-Recombinant-ATP2A2 antibody [RM1Q21] (STJA0024005)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal anti-Recombinant-Sarcoplasmic/Endoplasmic Reticulum Calcium Atpase 2 for use in IF, IHC and WB in Human and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IF/IHC/WB |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | RM1Q21 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Protein A/G purified from cell culture supernatant |
| Dilution Range | IF: 1:50-1:200 |
| Formulation | 0.01M PBS |
| Storage Instruction | Suitable for storage at +4°C between 1-2 weeks. For longer term store at-20°C for up to 12 months. |
Target Information
| Gene Symbol | ATP2A2 |
| Gene ID | 488 |
| Uniprot ID | AT2A2_HUMAN |
Additional Info
| Post Translational Modifications | Nitrated under oxidative stress. Nitration on the two tyrosine residues inhibits catalytic activity. Serotonylated on Gln residues by TGM2 in response to hypoxia, leading to its inactivation. |
| Function | This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Involved in autophagy in response to starvation. Upon interaction with VMP1 and activation, controls ER-isolation membrane contacts for autophagosome formation. Also modulates ER contacts with lipid droplets, mitochondria and endosomes. In coordination with FLVCR2 mediates heme-stimulated switching from mitochondrial ATP synthesis to thermogenesis. Isoform 2: Involved in the regulation of the contraction/relaxation cycle. Acts as a regulator of TNFSF11-mediated Ca(2+) signaling pathways via its interaction with TMEM64 which is critical for the TNFSF11-induced CREB1 activation and mitochondrial ROS generation necessary for proper osteoclast generation. Association between TMEM64 and SERCA2 in the ER leads to cytosolic Ca(2+) spiking for activation of NFATC1 and production of mitochondrial ROS, thereby triggering Ca(2+) signaling cascades that promote osteoclast differentiation and activation. |
| Protein Name | Sarcoplasmic/Endoplasmic Reticulum Calcium Atpase 2Serca2Sr Ca(2+-Atpase 2Calcium Pump 2Calcium-Transporting Atpase Sarcoplasmic Reticulum Type - Slow Twitch Skeletal Muscle IsoformEndoplasmic Reticulum Class 1/2 Ca(2+ Atpase |
| Database Links | Reactome: R-HSA-1912420Reactome: R-HSA-418359Reactome: R-HSA-5578775Reactome: R-HSA-936837 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinSarcoplasmic Reticulum MembraneColocalizes With Flvcr2 At The Mitochondrial-Er Contact Junction |
| Alternative Antibody Names | Anti-Sarcoplasmic/Endoplasmic Reticulum Calcium Atpase 2 antibodyAnti-Serca2 antibodyAnti-Sr Ca(2+-Atpase 2 antibodyAnti-Calcium Pump 2 antibodyAnti-Calcium-Transporting Atpase Sarcoplasmic Reticulum Type - Slow Twitch Skeletal Muscle Isoform antibodyAnti-Endoplasmic Reticulum Class 1/2 Ca(2+ Atpase antibodyAnti-ATP2A2 antibodyAnti-ATP2B antibody |
Information sourced from Uniprot.org