Anti-REC8 antibody (194-561) (STJ117199)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal REC8 (194-561) antibody for use in WB and ELISA in rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | Rec8 |
| Gene ID | 56739 |
| Uniprot ID | REC8_MOUSE |
| Immunogen Region | 194-561 |
| Immunogen Sequence | EVITLQEAEPIRMLQIEGEQ DLPEISRGDLELLIAEKDDA ILLEERQRGRLLRQRRASLP LDESREEPRALEGAGLVSAL SPPAPAQVEGIQEALPGQVF PPEVQKMTGWEPGALLTEVT PPQELRLPAPPSTEKRLPSL QRPLPRRHRRRQLLFWDKET QISREKFEEQLQTGAHCWEY PVAQPPKRMLTSPAELFRTP TLSGWLPPELLGLWTHCAQV PQRMLRQRPQLETEETVEE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 194-561 of mouse REC8 (NP_064386.2). |
Additional Info
| Tissue Specificity | Expressed primarily in the gonads. In the testis, expressed in pachytene spermatocytes and in spermatids. Not expressed in spermatogonia or somatic cells. In the ovary, expressed only in oocytes. Low levels also detected in a number of somatic tissues including thymus, lung, liver, kidney and small intestine. |
| Post Translational Modifications | Phosphorylated. |
| Function | Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II. |
| Protein Name | Meiotic Recombination Protein Rec8 HomologCohesin Rec8p |
| Database Links | |
| Cellular Localisation | NucleusChromosomeCentromereIn Meiotic ChromosomesLocalized Along Axial Elements In Prophase From The Leptotene To Diplotene StagesAt Later Prophase StagesDiakinesis And Metaphase ILocalized Along Interstitial Axes Of Chromosomes Including Both Centromere And Arm RegionsNo Longer Detected In Arm Regions In Anaphase I But Persists On Centromere Regions Until Metaphase Ii |
| Alternative Antibody Names | Anti-Meiotic Recombination Protein Rec8 Homolog antibodyAnti-Cohesin Rec8p antibodyAnti-Rec8 antibodyAnti-Mei8 antibodyAnti-Rec8L1 antibody |
Information sourced from Uniprot.org